FGSC #2718 Mating Types: A Species: Neurospora crassa
Genotype: qa-2;aro-9
Alleles: 326M237;Y325M6
Linkage Group(s): VIIR;IIR
Genetic Background: SL
Stock No. from Other Collection: 326M237.Y325
Depositor of Strain: MEC
Strain of Opposite Mating Type: 0
Reference: Jacobsen et al 1975 J Bacteriol 124:491-496
Reference: Jacobsen et al 1975 J Biol Chem 250:6008-6014
Lesion: aro-9 aromatic-9
Enzyme Name: dehydroquinase
Lesion Information for Marker:
      Linkage Group of aro-9: IIR
Marker description or requirements:
      Requires shikimic acid or a mixture of the four aromatic amino acids when combined with qa-1. Single mutant grows on minimal media.
Reference for: aro-9: 11005. Catcheside, D.E.A. et al 1985. MGG 199:446-451
Reference for: aro-9: 11008. Coggins et al 1987. Meth Enzymol 142:325-341
Lesion: qa-2 quinate-2
Enzyme Name: catabolic dehydroquinase
Lesion Information for Marker:
      Linkage Group of qa-2: VII
      Markers Left of qa-2: qa-4
      Markers Right ofqa-2: met-7 (0.02%)
Marker description or requirements:
      VII. In the qa cluster between qa-4 and met-7 (0.02%) (146). (884) Lacks catabolic dehydroquinase (148, 517, 884). Unable to use quinate or shikimate as the sole carbon source in the presence of aro-9, which results in the absence of biosynthetic dehydroquinase (885) (Fig. 21). aro-9;qa-2+ strains grow on minimal medium without supplement. qa-2 is conveniently scored as an aro auxotroph when aro-9 is present. qa-2+ cloned in pBR322 (pVK57) is expressed constitutively from its own promoter in E. coli(23, 388, 451); has been returned to Neurospora chromosomal sites by transformation (150). Allele M246 is stable (150).
Reference for: qa-2: 23. Alton, N. K. , J. A. Hautala, N. H. Giles, S. R. Kushner, and D. Vapnek. 1978. Transcription and translation in E. coli of hybrid plasmids containing the catabolic dehydro- quinase gene from N. crassa. Gene 4:241-259.
Reference for: qa-2: 146. Case. M. E. , and N. H. Giles. 1976. Gene order in the qa gene cluster of N. crassa. Mol. Gen. Genet. 147:83-89.
Reference for: qa-2: 148. Case, M. E. , J. A. Hautala, and N. H. Giles. 1977. Characterization of qa-2 mutants of N. crassa by genetic, enzymatic, and immunologicaltechniques. J. Bacteriol. 129:166-172.
Reference for: qa-2: 150. Case, M. E. , M. Schweizer, S. R. Kushner, and N. H. Giles. 1979. Efficient transformation of N. crassa utilizing hybrid plasmid DNA. Proc. Natl. Acad. Sci. U. S. A. 76:5259-5263.
Reference for: qa-2: 388. Giles, N. H. , N. K. Alton, M. E. Case, J. A. Hautala, J. W. Jacobson, S. R. Kushner, V. B. Patel, W. R. Reinert, P. Stroman, and D. Vapnek. 1978. The organization of the qa gene cluster in Neurospora crassa and its expression in Escherichia coli. Stadler Genet. Symp. 10:49-63.
Reference for: qa-2: 451. Hautala, J. A. , M. Schweizer, N. H. Giles, and S. R. Kushner. 1979. Constitutive expression in Escherichia coli of the Neurospora crassastructural gene encoding the inducible enzyme catabolic dehydroquinase. Mol. Gen. Genet. 172:93-98.
Reference for: qa-2: 517. Jacobson, J. W. , J. A. Hautala, M. E. Case, and N. H. Giles. 1975. Effect of mutations in the qa gene cluster of Neurospora crassa on theenzyme catabolic dehydroquinase. J. Bacteriol. 124:491-496.
Reference for: qa-2: 884. Rines, H. W. 1969. Genetical and biochemical studies on the inducible quinic acid catabolic pathway in Neurospora crassa. Ph. D. thesis,Yale University. Diss. Abstr. Int. 31:1088B-1089B (1970).
Reference for: qa-2: 885. Rines, H. W. , M. E. Case, and N. H. Giles. 1969. Mutants in the arom gene cluster of Neurospora crassa specific for biosyntheticdehydroquinase. Genetics 61:789-880.
Reference for: qa-2: 11015. Geever et al 1989. J Mol Biol 207:15-34
Reference for: qa-2: 11016. Giles et al 1985. Microbiol Rev 49:338-358


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