FGSC #2284 Mating Types: A Species: Neurospora crassa
Genotype: spco-4 wc-1 nt
Alleles: R2367 P829 65001
Linkage Group(s): VIIL VIIR VIIR
Stock No. from Other Collection: 7113
Depositor of Strain: DDP
Strain of Opposite Mating Type: 2285
Markers
Lesion: nt nicotinic acid or tryptophan
Enzyme Name: tryptophan pyrrolase
Lesion Information for Marker:
      Linkage Group of nt: VIIR
      Markers Left of nt: arg-10 (2 to 12%)
      Markers Right ofnt: sk (7 to 18%)
Marker description or requirements:
      VIIR. Between arg-10 (2 to 12%) and sk (7 to 18%) (789). (874) Uses nicotinic acid. May respond also to tryptophan, phenylalanine, tyrosine, quinic acid, and precursors of nicotinic acid or tryptophan, or both, depending on genetic background (448, 760). Best supplemented with nicotinamide and scored as a nic mutant. Probably deficient in tryptophan pyrrolase (tryptophan 2,3-dioxygenase) (Fig. 18), but direct evidence is lacking because tryptophan oxygenase cannot be assayed in Neurospora (368). Kynurenine formamidase levels are normal (368). Pyridine nucleotide levels (111).
Reference for: nt: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nt: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nt: 448. Haskins, F. H. , and H. K. Mitchell. 1952. An example of the influence of modifying genes in Neurospora. Am. Nat. 86:231-238.
Reference for: nt: 760. Newmeyer, D. , and E. L. Tatum. 1953. Gene expression in Neurospora mutants requiring nicotinic acid or tryptophan. Am. J. Bot. 40:393-400.
Reference for: nt: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nt: 874. Reisner, A. , R. W. Barratt, and D. Newmeyer. 1953. Confirmation of the seventh linkage group of Neurospora crassa. Genetics 38:685(Abstr. ).
Lesion: spco-4 spreading colonial-4
Lesion Information for Marker:
      Linkage Group of spco-4: VIIL
      Markers Left of spco-4: Linked to do (<1%) and nic-3 (1%, probably to the left)
Marker description or requirements:
      VIIL. Linked to do (<1%) and nic-3 (1%, probably to the left) (816). Fine hyphae (382). Initially aconidial. Capable of conidiating on the surface of complete medium (D.D. Perkins, unpublished data). Hyphae extend faster within agar medium than on the surface, resulting in a dense hemispherical colony, most of which is embedded (1085).
Reference for: spco-4: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: spco-4: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: spco-4: 1085. Trinci, A. P. J. 1973. Growth of wild type and spreading colonial mutants of Neurospora crassa in batch culture and on agar medium. Arch. Mikrobiol. 91:113-126.
Lesion: wc-1 white collar-1
Lesion Information for Marker:
      Linkage Group of wc-1: VIIR
      Markers Left of wc-1: met-9 (1 to 4%)
      Markers Right ofwc-1: un-10 (7%) and for (6%)
Marker description or requirements:
      VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).
Reference for: wc-1: 444. Harding, R. W. , and W. Shropshire, Jr. 1980. Photocontrol of carotenoid biosynthesis. Annu. Rev. Plant Physiol. 31:217-238.
Reference for: wc-1: 445. Harding, R. W. , and R. V. Turner. 1981. Photoregulation of the carotenoid biosynthetic pathway in albino and white collar mutants ofNeurospora crassa. Plant Physiol. 68:745-749.
Reference for: wc-1: 724. Murray, N. E. 1969. Reversal of polarized recombination of alleles in Neurospora as a function of their position. Genetics 61:67-77.
Reference for: wc-1: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: wc-1: 800. Perkins, D. D. 1972. Linkage testers having markers near the centromere. Neurospora Newsl. 19:33.
Reference for: wc-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: wc-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.

 

To order, please copy the strain number into the Material Request Form 

Back to Strain Search Form

To the FGSC home page

6/11/12