FGSC #2074 Mating Types: a Species: Neurospora crassa
Genotype: nic-3 wc-1 sk
Alleles: Y31881 P829 B234
Linkage Group(s): VIIL VIIR VIIR
Stock No. from Other Collection: 7102
Depositor of Strain: DDP
Strain of Opposite Mating Type: 2073
Markers
Lesion: nic-3 nicotinic acid-3
Lesion Information for Marker:
      Linkage Group of nic-3: VIIL
      Markers Left of nic-3: spco-4 (1%) and do (3%)
      Markers Right ofnic-3: thi-3 (9 to 27%) and csp-2 (16 to 22%)
Marker description or requirements:
      VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.
Reference for: nic-3: 96. Bonner, D. M. 1948. The identification of a natural precursor of nicotinic acid. Proc. Natl. Acad. Sci. U. S. A. 34:5-9.
Reference for: nic-3: 100. Bonner, D. M. , and C. Yanofsky. 1949. Quinolinic acid accumulation in the conversion of 3-hydroxyanthranilic acid to niacin inNeurospora. Proc. Natl. Acad. Sci. U. S. A. 35:576- 581.
Reference for: nic-3: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nic-3: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nic-3: 539. Kafer, E. , and E. Perlmutter. 1980. Isolation and genetic analysis of MMS-sensitive mus mutants of Neurospora. Can. J. Genet. Cytol. 22:535-552.
Reference for: nic-3: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: nic-3: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nic-3: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: nic-3: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: nic-3: 1168. Yanofsky, C. , and D. M. Bonner. 1950. Evidence for the participation of kynurenine as a normal intermediate in the biosynthesis of niacinin Neurospora. Proc. Natl. Acad. Sci. U. S. A. 36:167-176.
Lesion: sk skin
Lesion Information for Marker:
      Linkage Group of sk: VIIR
      Markers Left of sk: nt (7 to 17%)
Marker description or requirements:
      VIIR. Right of nt (7 to 17%) (789). Leathery, nonconidiating rapid surface growth (789). "Mucilaginous substrate hyphae" (1088). sk ascospores are slow to mature, but good allele ratios are obtained from crosses held for 3 weeks at 25°C. Female sterile. Allele R2466 called mo-3; alleles Y6821, R2408, and R2529 called moe-1(382, PB).
Reference for: sk: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: sk: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: sk: 1088. Turian, G. , and D. E. Bianchi. 1972. Conidiation in Neurospora. Bot. Rev. 38:119-154.
Lesion: wc-1 white collar-1
Lesion Information for Marker:
      Linkage Group of wc-1: VIIR
      Markers Left of wc-1: met-9 (1 to 4%)
      Markers Right ofwc-1: un-10 (7%) and for (6%)
Marker description or requirements:
      VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).
Reference for: wc-1: 444. Harding, R. W. , and W. Shropshire, Jr. 1980. Photocontrol of carotenoid biosynthesis. Annu. Rev. Plant Physiol. 31:217-238.
Reference for: wc-1: 445. Harding, R. W. , and R. V. Turner. 1981. Photoregulation of the carotenoid biosynthetic pathway in albino and white collar mutants ofNeurospora crassa. Plant Physiol. 68:745-749.
Reference for: wc-1: 724. Murray, N. E. 1969. Reversal of polarized recombination of alleles in Neurospora as a function of their position. Genetics 61:67-77.
Reference for: wc-1: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: wc-1: 800. Perkins, D. D. 1972. Linkage testers having markers near the centromere. Neurospora Newsl. 19:33.
Reference for: wc-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: wc-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.

 

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6/11/12