FGSC #1619 Mating Types: A Species: Neurospora crassa
Genotype: su(trp-3td201);trp-3
Alleles: su201-4;td201
Linkage Group(s): VIIR;IIR
Genetic Background: M
Mutagen: UV
Stock No. from Other Collection: su201-4
Depositor of Strain: SRS
Strain of Opposite Mating Type: 0
Reference: Yourno & Suskind Genetics 50:803-16 1964 XY2
Lesion: trp-3 tryptophan-3
Enzyme Name: tryptophan synthetase
Lesion Information for Marker:
      Linkage Group of trp-3: IIR
      Markers Left of trp-3: fl (2 to 6%)
      Markers Right oftrp-3: rip-1 (9%) and un-5(10%)
Marker description or requirements:
      IIR. Right of fl (2 to 6%). Left of rip-1 (9%) and un-5(10%) (816, PB). (1166). Uses tryptophan (685); strains carrying some alleles also use indole (4). Structural gene for tryptophan synthetase (1167), called tryptophan desmolase in early literature. Tryptophan synthetase catalyzes three reactions: indoleglycerol-phosphate -> tryptophan, indole tryptophan, and indoleglycerol-phosphate <-> indole (Fig. 11). In Neurospora, all three reactions are catalyzed by a single protein, which is specified by a single gene (645, 1167). Mutants lack indoleglycerol-phosphate -> tryptophan activity but differ with respect to the other activities; e.g., strains carrying trp-3 allele (td141 are blocked in indoleglycerol-phosphate utilization but can use indole; trp-3 (td100) can synthesize indole but not convert it to tryptophan; trp-3 (td140) lacks all three activities. (See references 582 and 1049 for citations and characteristics of other mutants.) Used extensively for studies of gene structure in relation to enzymatic activity (257, 582 and references therein, 1167). The active enzyme is a homooligomer (645) thought to have two domains (644 and references therein). Biochemical studies of complementation between alleles: in vivo (582, 583) and in vitro (1048 and references therein). Complementation maps (4, 5, 9 and references therein, 582). Fine-structure maps (5, 540, 582, 1049). Reviewed as example of gene fusion (218). trp-3 mutant C83 provided the first proved example in Neurospora of gene-controlled loss of enzyme activity (685); trp-3 mutant S1952 provided the first example of allele-specific suppression restoring functional wild-type-like enzyme (1166). Allele td140 is supersuppressible (954, 955). Certain classes of trp-3mutants are osmotic remediable (583). Called td and tryp-3.
Reference for: trp-3: 4. Ahmad, M. , and D. G. Catcheside. 1960. Physiological diversity amongst tryptophan mutants in N. crassa. Heredity 15:55-64.
Reference for: trp-3: 5. Ahmad, M. , M. K. U. Choudhury, and S. M. Islam. 1969. Complementation and recombination between indole utilising tryptophan-3 mutantsof N. crassa. Heredity 24:656-660.
Reference for: trp-3: 9. Ahmad, M. , and N. Islam. 1969. Interallelic complementation at the tryptophan-3 locus in N. crassa. Heredity 24:651-655.
Reference for: trp-3: 218. Crawford, I. P. 1980. Gene fusions in the tryptophan pathway:tryptophan synthetase and phosphoribosyl-anthranilate isomerase:indoleglycerolphosphate synthase. In Multifunctional Proteins. H. Bisswanger and E. Schmincke-Ott (ed. ). Wiley, New York.p. 151-173.
Reference for: trp-3: 257. De Moss, J. A. 1962. Studies on the mechanism of the tryptophan synthetase reaction. Biochim. Biophys. Acta 62:279-293.
Reference for: trp-3: 540. Kaplan, S. , Y. Suyama, and D. M. Bonner. 1964. Fine structure analysis at the td locus of Neurospora crassa. Genetics 49:145-158.
Reference for: trp-3: 582. Lacy, A. M. 1965. Structural and physiological relationships within the td locus in Neurospora crassa. Biochem. Biophys. Res. Comm. 18:812-823.
Reference for: trp-3: 583. Lacy, A. M. , S. Mellen, and K. Pomerance. 1968. Genetics and biochemistry of osmotic-remedial td mutants of Neurospora. Proc. 12thIntern. Congr. Genet. (Tokyo) 1:21 (Abstr.).
Reference for: trp-3: 644. Matchett, W. H. 1974. Indole channeling by tryptophan synthase of Neurospora. J. Biol. Chem. 249:4041-4049.
Reference for: trp-3: 645. Matchett, W. H. , and J. A. DeMoss. 1975. The subunit structure of tryptophan synthase from Neurospora crassa. J. Biol. Chem. 250:2941- 2946.
Reference for: trp-3: 685. Mitchell, H. K. , and J. Lein. 1948. A Neurospora mutant deficient in the enzymatic synthesis of tryptophan. J. Biol. Chem. 175:481- 482.
Reference for: trp-3: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: trp-3: 954. Seale, T. W. 1972. Super-suppressors in Neurospora crassa. I. Induction, genetic localization and relationship to a missense suppressor. Genetics 70:385-396.
Reference for: trp-3: 955. Seale, T. W. 1976. Super-suppressor action spectrum in Neurospora. Mol. Gen. Genet. 148:105-108.
Reference for: trp-3: 1048. Suyama, Y. , and D. M. Bonner. 1964. Complementation between tryptophan synthetase mutants of Neurospora crassa. Biochim. Biophys. Acta. 81:565-575.
Reference for: trp-3: 1049. Suyama, Y. , A. M. Lacy, and D. M. Bonner. 1964. A genetic map of the td locus of Neurospora crassa. Genetics 49:135-144.
Reference for: trp-3: 1166. Yanofsky, C. 1952. The effects of gene change on tryptophan desmolase formation. Proc. Natl. Acad. Sci. U. S. A. 38:215-226.
Reference for: trp-3: 1167. Yanofsky, C. 1960. The tryptophan synthetase system. Bacteriol. Rev. 24:221-245.
Lesion: su(trp-3td201) suppressor of (trp-3td201)


To order, please copy the strain number into the Material Request Form 

Back to Strain Search Form

To the FGSC home page