FGSC #A1232 Species: Aspergillus nidulans
Genotype: fwA1,pabaA1::alcA(p)cotA,pyr-4::argB,prnAGFP
Stock No. from Other Collection: NF1-1
Depositor of Strain: GT
Reference: Johns et al. Mol. Gen. Genomics (2006) 275 : 593-604
Markers
Lesion: pabaA1 p-aminobenzoic acid req.
Lesion Information for Marker:
      Linkage Group of pabaA1: IR
Marker description or requirements:
      Requires p-aminobenzoic acid
Reference for: pabaA1: 10536. Pontecorvo, G. et al. 1953. Adv. Genet. 5,141-238
Reference for: pabaA1: 10658. Timberlake, W. E. . unpublished
Lesion: pyr-4 pyrimidine-4
Enzyme Name: orotidine 5'-monophosphate decarboxylase
Lesion Information for Marker:
      Linkage Group of pyr-4: IIL
      Markers Left of pyr-4: het-c (1%), T(P2869), and cys-3 (18 to 21%)
      Markers Right ofpyr-4: ro-3 (1 to 2%)
Marker description or requirements:
      IIL. Right of het-c (1%), T(P2869), and cys-3 (18 to 21%). Left of ro-3 (1 to 2%) (721, 816, PB). (812). Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate decarboxylase (133, 134, 841) (Fig. 20). Fertile crosses homozygous for pyr-4 can be made by using very high levels of uridine (15 to 20 mg/ml) (O.M. Mylyk, personal communication).
Reference for: pyr-4: 133. Caroline, D. J. F. 1968. Pyrimidine synthesis in N. crassa. Ph. D. thesis, University of Michigan, Ann Arbor. Diss. Abstr. 30:960B.
Reference for: pyr-4: 134. Caroline, D. F. 1969. Pyrimidine synthesis in N. crassa:Gene-enzyme relationships. J. Bacteriol. 100:1371-1377.
Reference for: pyr-4: 721. Murray, N. E. 1965. Cysteine mutant strains of Neurospora. Genetics 52:801-808.
Reference for: pyr-4: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: pyr-4: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: pyr-4: 841. Pynadath, T. I. , and R. M. Fink. 1967. Studies of orotidine 5'-phosphate decarboxylase in Neurospora crassa. Arch. Biochem. Biophys. 118:185-189.

 

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