FGSC #1105 Mating Types: A Species: Neurospora crassa
Genotype: aro-4;inl
Alleles: R2219;89601
Linkage Group(s): IIR;VR
Genetic Background: M
Mutagen: UV
Stock No. from Other Collection: R2219
Depositor of Strain: MEC
Strain of Opposite Mating Type: 0
Lesion: aro-4 aromatic-4
Enzyme Name: 3-enolpyruvate shikimic acid-5 phosphate synthetase
Lesion Information for Marker:
      Linkage Group of aro-4: IIR
Marker description or requirements:
      requires a mix of trp+phe+tyr+paba
Reference for: aro-4: 11005. Catcheside, D.E.A. et al 1985. MGG 199:446-451
Reference for: aro-4: 11008. Coggins et al 1987. Meth Enzymol 142:325-341
Lesion: inl inositol
Enzyme Name: myoinositol-1-phosphatase
Lesion Information for Marker:
      Linkage Group of inl: VR
      Markers Left of inl: pho-3 (3 to 4%),and al-3
      Markers Right ofinl: pab-1 (1 to 10%)
Marker description or requirements:
      VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.
Reference for: inl: 65. Beadle, G. W. 1944. An inositolless mutant strain of Neurospora and its use in bioassays. J. Biol. Chem. 156:683-689.
Reference for: inl: 194. Chen, Y. F. , C. C. Ho, and A. L. Demain. 1976. Genetic improvement of production of amylases in Neurospora intermedia. Genetics 83:s14(Abstr).
Reference for: inl: 325. Fass, D. N. 1969. Glucamylase of Neurospora:a regulated exoenzyme. Ph. D. thesis, Florida State University. Diss. Abstr. Intern. 30:5375B(1970).
Reference for: inl: 362. Freese, E. 1957. Uber die Feinstruktur des Genoms im Bereich eines PAB Locus von Neurospora crassa. Z. Indukt. Abstamungs. -Vererbungsl. 88:388-406.
Reference for: inl: 366. Fuller, R. C. , R. W. Barratt, and E. L. Tatum. 1950. The relationship between hexachlorocyclohexane and inositol in Neurospora. J. Biol. Chem. 186:823-827.
Reference for: inl: 367. Fuller, R. C. , and E. L. Tatum. 1956. Inositol-phospholipid in Neurospora and its relationship to morphology. Am. J. Bot. 43:361-365.
Reference for: inl: 386. Giles, N. H. 1951. Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spring Harbor Symp. Quant. Biol. 16:283-313.
Reference for: inl: 390. Giles, N. H. , and C. W. H. Partridge. 1953. The effect of a suppressor on allelic inositolless mutants in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 39:479-488.
Reference for: inl: 392. Giles, N. H. , F. J. de Serres, and C. W. H. Partridge. 1955. Comparative studies of X-ray-induced forward and reverse mutation. Ann. N. Y. Acad. Sci. 59:536-552.
Reference for: inl: 397. Gleason, M. K. , and R. L. Metzenberg. 1974. Regulation of phosphate metabolism in Neurospora crassa:isolation of mutants deficient inthe repressible alkaline phosphatase. Genetics 78:645-659.
Reference for: inl: 439. Hanson, B. A. 1980. Inositol-limited growth, repair, and translocation in an inositol-requiring mutant of Neurospora crassa. J. Bacteriol. 143:18-26.
Reference for: inl: 440. Hanson, B. , and S. Brody. 1979. Lipid and cell wall changes in an inositol-requiring mutant of Neurospora crassa. J. Bacteriol. 138:461-466.
Reference for: inl: 457. Hitz, H. -R. 1963. Die Wirkung von Hexachlorcyclohexanen bei Neurospora crassa inositolless. Arch. Mikrobiol. 45:217-246.
Reference for: inl: 482. Houlahan, M. B. , G. W. Beadle, and H. G. Calhoun. 1949. Linkage studies with biochemical mutants of Neurospora crassa. Genetics 34:493-507.
Reference for: inl: 501. Hubbard, S. C. , and S. Brody. 1975. Glycerophospholipid variation in choline and inositol auxotrophs of Neurospora crassa:internalcompensation among zwitterionic and anionic species. J. Biol. Chem. 250:7173-7181.
Reference for: inl: 606. Lester, H. E. , and S. R. Gross. 1959. Efficient method for selection of auxotrophic mutants of Neurospora. Science 129:572.
Reference for: inl: 641. Marzluf, G. A. 1973. Regulation of sulfate transport in Neurospora by transinhibition and by inositol depletion. Arch. Biochem. Biophys. 156:244-254.
Reference for: inl: 647. Matile, P. 1966. Inositol deficiency resulting in death:an explanation of its occurrence in Neurospora crassa. Science 151:86- 88.
Reference for: inl: 677. Mishra, N. C. , G. Szabo, and E. L. Tatum. 1973. Nucleic acid-induced genetic changes in Neurospora. In M. C. Niu and S. J. Segal (ed. ), The role of RNA in reproduction and development. North/Holland, Amsterdam.p. 259-268.
Reference for: inl: 679. Mishra, N. C. , and E. L. Tatum. 1973. Non-Mendelian inheritance of DNA-induced inositol independence in Neurospora. Proc. Natl. Acad. Sci. U. S. A. 70:3875-3879.
Reference for: inl: 702. Munkres, K. D. 1981. Biochemical genetics of aging of Neurospora crassa and Podospora anserina:a review. In R. S. Sohal (ed. ),Age pigments. North-Holland, Amsterdam.p. 83-100.
Reference for: inl: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: inl: 826. Pina, E. , and E. L. Tatum. 1967. Inositol biosynthesis in Neurospora crassa. Biochim. Biophys. Acta 136:265-271.
Reference for: inl: 832. Pittenger, T. H. , and D. J. West. 1979. Isolation and characterization of temperature-sensitive respiratory mutants of Neurospora crassa. Genetics 93:539-555.
Reference for: inl: 917. Sargent, M. L. , and S. H. Kaltenborn. 1972. Effects of medium composition and carbon dioxide on circadian conidiation in Neurospora. Plant Physiol. 50:171-175.
Reference for: inl: 931. Schopfer, W. H. , and T. Posternak. 1958. Action d'anti-inositols sur Neurospora crassa "inositolless" cultive en milieu hautement purifie. Arch. Mikrobiol. 31:240-243.
Reference for: inl: 1027. Sternberg, D. , and A. S. Sussman. 1974. Hyperproduction of some glycosidases in Neurospora crassa. Arch. Microbiol. 101:303-320.
Reference for: inl: 1028. Stevens, C. M. , and A. Mylroie. 1953. Inhibition effects in back-mutation tests with mutants of Neurospora. Nature 171:179-180.
Reference for: inl: 1033. Strauss, B. S. 1958. Cell death and "unbalanced growth" in Neurospora. J. Gen. Microbiol. 18:658-669.
Reference for: inl: 1036. Strickland, W. N. , D. D. Perkins, and C. C. Veatch. 1959. Linkage data for group V markers in Neurospora. Genetics 44:1221-1226.
Reference for: inl: 1043. Sullivan, J. L. , and A. G. DeBusk. 1971. Method for specific selection of temperature-sensitive mutants. Neurospora Newsl. 18:13.
Reference for: inl: 1142. Williams, S. G. 1971. Biosynthesis of inositol by inositol-less mutants of Neurospora crassa. Aust. J. Biol. Sci. 24:1181-1188.
Reference for: inl: 1162. Wootton, J. C. , M. J. Fraser, and A. J. Baron. 1980. Efficient transformation of germinating Neurospora conidia using total nuclear DNAfragments. Neurospora Newsl. 27:33.
Reference for: inl: 1183. Zsindely, A. , M. Szabolcs, M. Kavai, M. Schablik, J. Aradi, and G. Szabo. 1979. Demonstration of myo-inositol1-phosphate synthase andits assumed defective variant in various Neurospora crassa strains by immunological methods. Acta Biol. Acad. Sci. Hung. 30:141-149.


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