FGSC #576 Mating Types: A Species: Neurospora crassa
Genotype: sfo nt
Alleles: E18829(d) 65001
Linkage Group(s): VIIC VIIR
Stock No. from Other Collection: E-18558-9(22
Depositor of Strain: ELT
Strain of Opposite Mating Type: 506
Markers
Lesion: nt nicotinic acid or tryptophan
Enzyme Name: tryptophan pyrrolase
Lesion Information for Marker:
      Linkage Group of nt: VIIR
      Markers Left of nt: arg-10 (2 to 12%)
      Markers Right ofnt: sk (7 to 18%)
Marker description or requirements:
      VIIR. Between arg-10 (2 to 12%) and sk (7 to 18%) (789). (874) Uses nicotinic acid. May respond also to tryptophan, phenylalanine, tyrosine, quinic acid, and precursors of nicotinic acid or tryptophan, or both, depending on genetic background (448, 760). Best supplemented with nicotinamide and scored as a nic mutant. Probably deficient in tryptophan pyrrolase (tryptophan 2,3-dioxygenase) (Fig. 18), but direct evidence is lacking because tryptophan oxygenase cannot be assayed in Neurospora (368). Kynurenine formamidase levels are normal (368). Pyridine nucleotide levels (111).
Reference for: nt: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nt: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nt: 448. Haskins, F. H. , and H. K. Mitchell. 1952. An example of the influence of modifying genes in Neurospora. Am. Nat. 86:231-238.
Reference for: nt: 760. Newmeyer, D. , and E. L. Tatum. 1953. Gene expression in Neurospora mutants requiring nicotinic acid or tryptophan. Am. J. Bot. 40:393-400.
Reference for: nt: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nt: 874. Reisner, A. , R. W. Barratt, and D. Newmeyer. 1953. Confirmation of the seventh linkage group of Neurospora crassa. Genetics 38:685(Abstr. ).
Lesion: sfo sulfonamide dependent
Lesion Information for Marker:
      Linkage Group of sfo: VII
      Markers Left of sfo: thi-3 (6%)
      Markers Right ofsfo: hlp-1(1 to 9%);Linked to the centromere (<1%)
Marker description or requirements:
      Linked to the centromere (<1%) (318), between thi-3 (6%) (874) and hlp-1(1 to 9%) (458). Requires sulfonamide at 35°C and is stimulated by sulfonamide at lower temperatures (319). Overproduces p-aminobenzoic acid; hence, growth is inhibited by exogenous p-aminobenzoic acid (1179). For sfo;pab-1 double mutant on minimal medium plus sulfonamide, p-amino-benzoic acid is stimulatory at very low concentrations but inhibitory at higher (319). For sfo;met-1 double mutant on minimal medium plus sulfonamide, methionine is stimulatory at very low concentrations but inhibitory at higher (1180). Best tested on solid minimal medium growth tubes at 35°C. Suppressor mutations occur frequently (318).
Reference for: sfo: 318. Emerson, M. R. , and S. Emerson. 1951. The sulfonamiderequiring character (sfo) as a marker for chromosome VII of Neurospora crassa. Microb. Genet. Bull. 4:7-9.
Reference for: sfo: 319. Emerson, S. 1947. Growth responses of a sulfonamide-requiring mutant strain of Neurospora. J. Bacteriol. 54:195-207.
Reference for: sfo: 458. Ho, C. C. 1969. Mutants of Neurospora crassa permeable to histidinol. Genetics 62:725-733.
Reference for: sfo: 874. Reisner, A. , R. W. Barratt, and D. Newmeyer. 1953. Confirmation of the seventh linkage group of Neurospora crassa. Genetics 38:685(Abstr. ).
Reference for: sfo: 1179. Zalokar, M. 1948. The p-amino benzoic acid requirement of the "sulfonamide-requiring" mutant strain of Neurospora. Proc. Natl. Acad. Sci. U. S. A. 34:32-36.
Reference for: sfo: 1180. Zalokar, M. 1950. The sulfanomide-requiring mutant of Neurospora:threonine-methionine antagonism. J. Bacteriol. 60:191-203.

 

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6/11/12