FGSC #9454 Species: Neurospora crassa
Genotype: col-4;gna-1
Alleles: 70007c, ?
Linkage Group(s): IVR, IIIR
Stock No. from Other Collection: OG89
Depositor of Strain: OG
Lesion: col-4 colonial-4
Lesion Information for Marker:
      Linkage Group of col-4: IVR
      Markers Left of col-4: met-1 (4%)
      Markers Right ofcol-4: arg-2 (<1 to 2%)
Marker description or requirements:
      IVR. Between met-1 (4%) and arg-2 (<1 to 2%) (692, 876, 991). (695) Spreading colonial morphology, forming dense balls of conidia high in slants (47). Probably dominant in heterozygous duplications from T(S1229) (E.G. Barry, personal communication). Cell wall-autolyzing enzyme (631). Reduced amount of cell wall peptides (1165). Used in combination with pe fl to produce microconidiating colonial growth suitable for reversion experiments (386). Called spco-1 (382); called c (386).
Reference for: col-4: 47. Barratt, R. W. , D. Newmeyer, D. D. Perkins, and L. Garnjobst. 1954. Map construction in N. crassa. Adv. Genet. 6:1-93.
Reference for: col-4: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: col-4: 386. Giles, N. H. 1951. Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spring Harbor Symp. Quant. Biol. 16:283-313.
Reference for: col-4: 631. Mahadevan, P. R. , and U. R. Mahadkar. 1970. Role of enzymes in growth and morphology of Neurospora crassa:cell-wall-bound enzymesand their possible role in branching. J. Bacteriol. 101:941-947.
Reference for: col-4: 692. Mitchell, M. B. , and H. K. Mitchell. 1954. A partial map of linkage group D in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 40:436-440.
Reference for: col-4: 695. Mitchell, M. B. , T. H. Pittenger, and H. K. Mitchell. 1952. Pseudo-wild types in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 38:569-580.
Reference for: col-4: 876. Reissig, J. L. 1960. Forward and back mutation in the pyr-3 region of Neurospora. I. Mutations from arginine dependence to prototrophy. Genet. Res. 1:356-374.
Reference for: col-4: 991. Smith, B. R. 1962. Linkage data for group IV. Neurospora Newsl. 1:16-17.
Reference for: col-4: 1165. Wrathall, C. R. , and E. L. Tatum. 1974. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem. Genet. 12:59-68.
Lesion: gna-1 guanine nucleotide-alpha-1
Lesion Information for Marker:
      Linkage Group of gna-1: IIIR
Marker description or requirements:
      IIIR. Linked to ro-2 (0/17). Encodes guanine nucleotide-binding protein alpha-1-subunit. The predicted amino acid sequence shows significant homology to the Gi family found in higher organisms. The null mutant shows female infertility, osmotic sensitivity, reduced hyphal growth rate, and defective macroconidiation, has decreased intracellular cAMP levels, and is more resistant to some environmental stresses than wild type. Mutants that are predicted to maintain an activated state of GNA-1 have increased intracellular cAMP levels, overproduce aerial hyphae, and are more sensitive to environmental stresses than wild type. Null-mutant extracts have reduced Mg^2+ -dependent adenylyl cyclase and cAMP phosphodiesterase activity. Mg^2+ -dependent adenylyl cyclase activity in wild-type extracts can be inhibited by using antibody against GNA-1.


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