FGSC #9442 Species: Neurospora crassa
Genotype: cot-1;col-17
Alleles: C102(t),B5
Linkage Group(s): IVR,VII
Stock No. from Other Collection: OG77
Depositor of Strain: OG
Lesion: col-17 colonial-17
Lesion Information for Marker:
      Linkage Group of col-17: VII
      Markers Left of col-17: Linked to nt (14%) and spco-5 (6%)
Marker description or requirements:
      VII. Linked to nt (14%) and spco-5 (6%) (382). Colonies grow very slowly (382).
Reference for: col-17: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Lesion: cot-1 colonial temperature sensitive-1
Lesion Information for Marker:
      Linkage Group of cot-1: IVR
      Markers Left of cot-1: pan-1 (2%)
      Markers Right ofcot-1: his-4 (1 to 6%)
Marker description or requirements:
      IVR. Between pan-1 (2%) and his-4 (1 to 6%) (692, 812, 816). Extremely colonial at 34°C, but completely normal growth, morphology, and fertility at 25°C and below. Linear growth is maximum at 24°C (374). Becomes colonial at 32°C; colonies from ascospores or conidia are viable and continue to grow slowly with dense branching, but do not conidiate. They quickly resume normal growth when shifted to a permissive temperature (692, 1068). Recessive in duplications (808); apparent dominance in heterokaryons (374) may have resulted from a shift in nuclear ratios. Used in studies of septation and branching (202), growth-inhibiting mucopolysaccharide (878, 879), and sulfate transport (641). Cell wall analysis (374). Growth is stimulated by lysine or arginine (0.1 mM) on glucose media at high temperatures (615). Because of high viability and tightly restricted growth at restrictive temperatures and normality at 25°C, cot-1 mutants have valuable technical applications. For example, crosses homozygous for cot-1 have been used in combination with sorbose for experiments with rec genes, where high-density ascospore platings are required for precise quantitative analysis of intralocus recombination (e.g., references 165, 997, and 1070). In another application, when shifted up after initial growth at the permissive low temperature, cot-1hyphae assume a "bottle brush" appearance with small side branches (692). This has been used to select uvs mutants by subsurface survival on UV-irradiated plates containing p-aminobenzoic acid (938; D.E.A. Catcheside, personal communication). cot-1 conidia or ascospores from cot-1 x cot-1crosses are used for replication in a protocol involving transfer by filter paper (615). For suppressors of cot-1, see gul.
Reference for: cot-1: 165. Catcheside, D. G. 1966. A second gene controlling allelic recombination in N. crassa. Aust. J. Biol. Sci. 19:1039-1046.
Reference for: cot-1: 202. Collinge, A. J. , M. H. Fletcher, and A. P. J. Trinci. 1978. Physiology and cytology of septation and branching in a temperature-sensitivecolonial mutant (cot-1) of N. crassa. Trans. Brit. Mycol. Soc. 71:107-120.
Reference for: cot-1: 374. Galsworthy, S. B. 1966. Biochemical aspects of temperature sensitivity in Neurospora. Ph. D. thesis, University of Wisconsin. Diss. Abstr. 26:6348.
Reference for: cot-1: 615. Littlewood, R. K. , and K. D. Munkres. 1972. A simple and reliable method for replica plating Neurospora crassa. J. Bacteriol. 110:1017-1021.
Reference for: cot-1: 641. Marzluf, G. A. 1973. Regulation of sulfate transport in Neurospora by transinhibition and by inositol depletion. Arch. Biochem. Biophys. 156:244-254.
Reference for: cot-1: 692. Mitchell, M. B. , and H. K. Mitchell. 1954. A partial map of linkage group D in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 40:436-440.
Reference for: cot-1: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: cot-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: cot-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: cot-1: 878. Reissig, J. L. 1974. Decoding of regulatory signals at the microbial surface. Curr. Top. Microbiol. Immunol. 67:43-96.
Reference for: cot-1: 879. Reissig, J. L. , and J. E. Glasgow. 1971. Mucopolysaccharide which regulates growth in Neurospora. J. Bacteriol. 106:882-889.
Reference for: cot-1: 938. Schroeder, A. L. , and L. D. Olson. 1980. Mutagen sensitive mutants in Neurospora, In F. J. de Serres, W. M. Generoso, and M. D. Shelbey (Ed. ), Conference on DNA Repair and Mutagenesis in Eukaryotes. Plenum Press, New York.p. 55-62.
Reference for: cot-1: 997. Smyth, D. R. 1971. Effect of rec-3 on polarity of recombination in the amination-1 locus of Neurospora crassa. Aust. J. Biol. Sci. 24:97-106.
Reference for: cot-1: 1068. Terenzi, H. F. , and J. L. Reissig. 1967. Modifiers of the cot gene in Neurospora:the gulliver mutants. Genetics 56:321-329.
Reference for: cot-1: 1070. Thomas, P. C. , and D. G. Catcheside. 1969. Genetic control of flanking marker behaviour in an allelic cross of Neurospora crassa. Can. J. Genet. Cytol. 11:558-566.


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