FGSC #9441 Species: Neurospora crassa
Genotype: cot-1;fr
Alleles: C102(t),B110
Linkage Group(s): IVR,IL
Stock No. from Other Collection: OG76
Depositor of Strain: OG
Lesion: cot-1 colonial temperature sensitive-1
Lesion Information for Marker:
      Linkage Group of cot-1: IVR
      Markers Left of cot-1: pan-1 (2%)
      Markers Right ofcot-1: his-4 (1 to 6%)
Marker description or requirements:
      IVR. Between pan-1 (2%) and his-4 (1 to 6%) (692, 812, 816). Extremely colonial at 34°C, but completely normal growth, morphology, and fertility at 25°C and below. Linear growth is maximum at 24°C (374). Becomes colonial at 32°C; colonies from ascospores or conidia are viable and continue to grow slowly with dense branching, but do not conidiate. They quickly resume normal growth when shifted to a permissive temperature (692, 1068). Recessive in duplications (808); apparent dominance in heterokaryons (374) may have resulted from a shift in nuclear ratios. Used in studies of septation and branching (202), growth-inhibiting mucopolysaccharide (878, 879), and sulfate transport (641). Cell wall analysis (374). Growth is stimulated by lysine or arginine (0.1 mM) on glucose media at high temperatures (615). Because of high viability and tightly restricted growth at restrictive temperatures and normality at 25°C, cot-1 mutants have valuable technical applications. For example, crosses homozygous for cot-1 have been used in combination with sorbose for experiments with rec genes, where high-density ascospore platings are required for precise quantitative analysis of intralocus recombination (e.g., references 165, 997, and 1070). In another application, when shifted up after initial growth at the permissive low temperature, cot-1hyphae assume a "bottle brush" appearance with small side branches (692). This has been used to select uvs mutants by subsurface survival on UV-irradiated plates containing p-aminobenzoic acid (938; D.E.A. Catcheside, personal communication). cot-1 conidia or ascospores from cot-1 x cot-1crosses are used for replication in a protocol involving transfer by filter paper (615). For suppressors of cot-1, see gul.
Reference for: cot-1: 165. Catcheside, D. G. 1966. A second gene controlling allelic recombination in N. crassa. Aust. J. Biol. Sci. 19:1039-1046.
Reference for: cot-1: 202. Collinge, A. J. , M. H. Fletcher, and A. P. J. Trinci. 1978. Physiology and cytology of septation and branching in a temperature-sensitivecolonial mutant (cot-1) of N. crassa. Trans. Brit. Mycol. Soc. 71:107-120.
Reference for: cot-1: 374. Galsworthy, S. B. 1966. Biochemical aspects of temperature sensitivity in Neurospora. Ph. D. thesis, University of Wisconsin. Diss. Abstr. 26:6348.
Reference for: cot-1: 615. Littlewood, R. K. , and K. D. Munkres. 1972. A simple and reliable method for replica plating Neurospora crassa. J. Bacteriol. 110:1017-1021.
Reference for: cot-1: 641. Marzluf, G. A. 1973. Regulation of sulfate transport in Neurospora by transinhibition and by inositol depletion. Arch. Biochem. Biophys. 156:244-254.
Reference for: cot-1: 692. Mitchell, M. B. , and H. K. Mitchell. 1954. A partial map of linkage group D in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 40:436-440.
Reference for: cot-1: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: cot-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: cot-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: cot-1: 878. Reissig, J. L. 1974. Decoding of regulatory signals at the microbial surface. Curr. Top. Microbiol. Immunol. 67:43-96.
Reference for: cot-1: 879. Reissig, J. L. , and J. E. Glasgow. 1971. Mucopolysaccharide which regulates growth in Neurospora. J. Bacteriol. 106:882-889.
Reference for: cot-1: 938. Schroeder, A. L. , and L. D. Olson. 1980. Mutagen sensitive mutants in Neurospora, In F. J. de Serres, W. M. Generoso, and M. D. Shelbey (Ed. ), Conference on DNA Repair and Mutagenesis in Eukaryotes. Plenum Press, New York.p. 55-62.
Reference for: cot-1: 997. Smyth, D. R. 1971. Effect of rec-3 on polarity of recombination in the amination-1 locus of Neurospora crassa. Aust. J. Biol. Sci. 24:97-106.
Reference for: cot-1: 1068. Terenzi, H. F. , and J. L. Reissig. 1967. Modifiers of the cot gene in Neurospora:the gulliver mutants. Genetics 56:321-329.
Reference for: cot-1: 1070. Thomas, P. C. , and D. G. Catcheside. 1969. Genetic control of flanking marker behaviour in an allelic cross of Neurospora crassa. Can. J. Genet. Cytol. 11:558-566.
Lesion: fr frost
Enzyme Name: glucose-6-phosphate dehydrogenase
Lesion Information for Marker:
      Linkage Group of fr: IL
      Markers Left of fr: ro-10 (18%)
      Markers Right offr: un-5 (6%)
Marker description or requirements:
      IL. Between ro-10 (18%) and un-5 (6%) (798, PB). (789)Delicate branching on agar surface and delicate aerial growth with no conidia (789). Multiple hyphal branching (382). Deficient in glucose- 6-phosphate dehydrogenase (as are col-2 and balmutants) (949, 952). Partially deficient in linolenic acid (115); morphology partially corrected by exogenous linolenic acid (892, 943). Low adenylate cyclase activity and low adenosine 3',5'-phosphate (943, 950). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Unlike cr-1, fr is not corrected morphologically by exogenous cyclic nucleotides (892, 951). Scott (943) reported that morphology is corrected by theophylline; Rosenberg and Pall (892) reported no correction by phosphodiesterase inhibitors. Cell wall analysis; photograph (112, 278, 946). Reduced amount of cell wall peptides (1165). Recessive in duplications (808). Female sterile. Both known alleles (B110 and R2499) revert to fr+.
Reference for: fr: 112. Brody, S. 1973. Metabolism, cell walls, and morphogenesis. In S. J. Coward (ed. ), Developmental regulation:aspects of celldifferentiation. Academic Press, New York.p. 107-154.
Reference for: fr: 115. Brody, S. and J. F. Nyc. 1970. Altered fatty acid distribution in mutants of N. crassa. J. Bacteriol. 104:780-786.
Reference for: fr: 278. De Terra, N. , and E. L. Tatum. 1963. A relationship between cell wall structure and colonial growth in Neurospora crassa. Am. J. Bot. 50:669-677.
Reference for: fr: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: fr: 779. Pall, M. L. , J. M. Trevillyan, and N. Hinman. 1981. Deficient cyclic adenosine 3',5'-monophosphate control in mutants of two genes ofNeurospora crassa. Mol. Cell. Biol. 1:1-8.
Reference for: fr: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: fr: 798. Perkins, D. D. 1972. An insertional translocation in Neurospora that generates duplications heterozygous for mating type. Genetics 71:25-51.
Reference for: fr: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: fr: 892. Rosenberg, G. , and M. L. Pall. 1979. Properties of two cyclic nucleotide-deficient mutants of Neurospora crassa. J. Bacteriol. 137:1140-1144.
Reference for: fr: 943. Scott, W. A. 1976. Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 73:2995-2999.
Reference for: fr: 946. Scott, W. A. 1979. Biochemical-genetic control of morphogenesis. In J. G. Scandalios (ed. ), Physiological genetics. AcademicPress, New York.p. 141-170.
Reference for: fr: 949. Scott, W. A. , and E. Mahoney. 1976. Defects of glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Neurospora and theirpleiotropic effects. Curr. Top. Cell Regul. 10:205-236.
Reference for: fr: 950. Scott, W. A. , N. C. Mishra, and E. L. Tatum. 1974. Biochemical genetics of morphogenesis in Neurospora. Brookhaven Symp. Biol. 25:1-18.
Reference for: fr: 951. Scott, W. A. and B. Solomon. 1975. Adenosine 3',5'-cyclic monophosphate and morphology in Neurospora crassa:drug-inducedalterations. J. Bacteriol. 122:454-463.
Reference for: fr: 952. Scott, W. A. , and E. L. Tatum. 1970. Glucose-6-phosphate dehydrogenase and Neurospora morphology. Proc. Natl. Acad. Sci. U. S. A. 66:515-522.
Reference for: fr: 1165. Wrathall, C. R. , and E. L. Tatum. 1974. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem. Genet. 12:59-68.


To order, please copy the strain number into the Material Request Form 

Back to Strain Search Form

To the FGSC home page