FGSC #9395 Species: Neurospora crassa
Genotype: col-4;fr
Alleles: 70007c,B110
Linkage Group(s): IVR,IL
Stock No. from Other Collection: OG30
Depositor of Strain: OG
Markers
Lesion: col-4 colonial-4
Lesion Information for Marker:
      Linkage Group of col-4: IVR
      Markers Left of col-4: met-1 (4%)
      Markers Right ofcol-4: arg-2 (<1 to 2%)
Marker description or requirements:
      IVR. Between met-1 (4%) and arg-2 (<1 to 2%) (692, 876, 991). (695) Spreading colonial morphology, forming dense balls of conidia high in slants (47). Probably dominant in heterozygous duplications from T(S1229) (E.G. Barry, personal communication). Cell wall-autolyzing enzyme (631). Reduced amount of cell wall peptides (1165). Used in combination with pe fl to produce microconidiating colonial growth suitable for reversion experiments (386). Called spco-1 (382); called c (386).
Reference for: col-4: 47. Barratt, R. W. , D. Newmeyer, D. D. Perkins, and L. Garnjobst. 1954. Map construction in N. crassa. Adv. Genet. 6:1-93.
Reference for: col-4: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: col-4: 386. Giles, N. H. 1951. Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spring Harbor Symp. Quant. Biol. 16:283-313.
Reference for: col-4: 631. Mahadevan, P. R. , and U. R. Mahadkar. 1970. Role of enzymes in growth and morphology of Neurospora crassa:cell-wall-bound enzymesand their possible role in branching. J. Bacteriol. 101:941-947.
Reference for: col-4: 692. Mitchell, M. B. , and H. K. Mitchell. 1954. A partial map of linkage group D in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 40:436-440.
Reference for: col-4: 695. Mitchell, M. B. , T. H. Pittenger, and H. K. Mitchell. 1952. Pseudo-wild types in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 38:569-580.
Reference for: col-4: 876. Reissig, J. L. 1960. Forward and back mutation in the pyr-3 region of Neurospora. I. Mutations from arginine dependence to prototrophy. Genet. Res. 1:356-374.
Reference for: col-4: 991. Smith, B. R. 1962. Linkage data for group IV. Neurospora Newsl. 1:16-17.
Reference for: col-4: 1165. Wrathall, C. R. , and E. L. Tatum. 1974. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem. Genet. 12:59-68.
Lesion: fr frost
Enzyme Name: glucose-6-phosphate dehydrogenase
Lesion Information for Marker:
      Linkage Group of fr: IL
      Markers Left of fr: ro-10 (18%)
      Markers Right offr: un-5 (6%)
Marker description or requirements:
      IL. Between ro-10 (18%) and un-5 (6%) (798, PB). (789)Delicate branching on agar surface and delicate aerial growth with no conidia (789). Multiple hyphal branching (382). Deficient in glucose- 6-phosphate dehydrogenase (as are col-2 and balmutants) (949, 952). Partially deficient in linolenic acid (115); morphology partially corrected by exogenous linolenic acid (892, 943). Low adenylate cyclase activity and low adenosine 3',5'-phosphate (943, 950). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Unlike cr-1, fr is not corrected morphologically by exogenous cyclic nucleotides (892, 951). Scott (943) reported that morphology is corrected by theophylline; Rosenberg and Pall (892) reported no correction by phosphodiesterase inhibitors. Cell wall analysis; photograph (112, 278, 946). Reduced amount of cell wall peptides (1165). Recessive in duplications (808). Female sterile. Both known alleles (B110 and R2499) revert to fr+.
Reference for: fr: 112. Brody, S. 1973. Metabolism, cell walls, and morphogenesis. In S. J. Coward (ed. ), Developmental regulation:aspects of celldifferentiation. Academic Press, New York.p. 107-154.
Reference for: fr: 115. Brody, S. and J. F. Nyc. 1970. Altered fatty acid distribution in mutants of N. crassa. J. Bacteriol. 104:780-786.
Reference for: fr: 278. De Terra, N. , and E. L. Tatum. 1963. A relationship between cell wall structure and colonial growth in Neurospora crassa. Am. J. Bot. 50:669-677.
Reference for: fr: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: fr: 779. Pall, M. L. , J. M. Trevillyan, and N. Hinman. 1981. Deficient cyclic adenosine 3',5'-monophosphate control in mutants of two genes ofNeurospora crassa. Mol. Cell. Biol. 1:1-8.
Reference for: fr: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: fr: 798. Perkins, D. D. 1972. An insertional translocation in Neurospora that generates duplications heterozygous for mating type. Genetics 71:25-51.
Reference for: fr: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: fr: 892. Rosenberg, G. , and M. L. Pall. 1979. Properties of two cyclic nucleotide-deficient mutants of Neurospora crassa. J. Bacteriol. 137:1140-1144.
Reference for: fr: 943. Scott, W. A. 1976. Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 73:2995-2999.
Reference for: fr: 946. Scott, W. A. 1979. Biochemical-genetic control of morphogenesis. In J. G. Scandalios (ed. ), Physiological genetics. AcademicPress, New York.p. 141-170.
Reference for: fr: 949. Scott, W. A. , and E. Mahoney. 1976. Defects of glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Neurospora and theirpleiotropic effects. Curr. Top. Cell Regul. 10:205-236.
Reference for: fr: 950. Scott, W. A. , N. C. Mishra, and E. L. Tatum. 1974. Biochemical genetics of morphogenesis in Neurospora. Brookhaven Symp. Biol. 25:1-18.
Reference for: fr: 951. Scott, W. A. and B. Solomon. 1975. Adenosine 3',5'-cyclic monophosphate and morphology in Neurospora crassa:drug-inducedalterations. J. Bacteriol. 122:454-463.
Reference for: fr: 952. Scott, W. A. , and E. L. Tatum. 1970. Glucose-6-phosphate dehydrogenase and Neurospora morphology. Proc. Natl. Acad. Sci. U. S. A. 66:515-522.
Reference for: fr: 1165. Wrathall, C. R. , and E. L. Tatum. 1974. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem. Genet. 12:59-68.

 

To order, please copy the strain number into the Material Request Form 

Back to Strain Search Form

To the FGSC home page

6/11/12