FGSC #9374 Species: Neurospora crassa
Genotype: spco-4;pk
Alleles: R2367,B6
Linkage Group(s): VIIL,VR
Stock No. from Other Collection: OG9
Depositor of Strain: OG
Lesion: pk peak (synonym
Enzyme Name: L-glutamine D-fructose-6-phosphate amidotransferase
Marker description or requirements:
      bis, biscuit) VR. Between met-3 (1%) and T(EB4)^L, cot-2 (8%), cl (2%). Growth on an agar surface is initially colonial and flat. A mass of aerial hyphae is then sent up, which conidiates profusely (1548). Somewhat similar in morphology to sn, cum, sp, and cot-4 at 25ºC, but distinguishable. Hyphae branch dichotomously (1405, 1548). Increased activity of L-glutamine:D-fructose-6-phosphate amidotransferase was observed in crude extracts of one pk strain, but not in nine others; increased activity for this enzyme also was found in cl and in four other nonallelic morphological mutants (1758). Hexoseaminoglycan consists of a single component on medium without sorbose, in contrast to two components in wild type (1960). Antigenic surface mucopolyoside (532). Cell-wall analysis and photograph, allele B6 (507) and allele C-1810-1 (287). Cell-wall enzymes (633). Effect of carbon source (531). One observation suggested a functional interaction with cl (1965), but substantial crossing-over frequencies and recovery of the double mutant pk cl indicated that the loci are distinct (569). The gene was named for the vegetative mutant phenotype, which is recessive. Several alleles were called bis (1592). The sexual phase is also affected. Asci are thin-walled, bulbous, and nonlinear in homozygous pk ´ pk crosses (1406, 1409, 1550). Spindle orientation is abnormal in the swollen asci, and no apical pore is formed (1625, 1679). Most mutant alleles are recessive for the ascus effect, but some are dominant with variable penetrance. Sorbose-resistant mutants at various loci act as dominance modifiers of the ascus effect of dominant alleles (1757). Sexual-phase-recessive allele C-1610 and dominant allele 17-088 are both associated with reciprocal translocations (1578).
Lesion: spco-4 spreading colonial-4
Lesion Information for Marker:
      Linkage Group of spco-4: VIIL
      Markers Left of spco-4: Linked to do (<1%) and nic-3 (1%, probably to the left)
Marker description or requirements:
      VIIL. Linked to do (<1%) and nic-3 (1%, probably to the left) (816). Fine hyphae (382). Initially aconidial. Capable of conidiating on the surface of complete medium (D.D. Perkins, unpublished data). Hyphae extend faster within agar medium than on the surface, resulting in a dense hemispherical colony, most of which is embedded (1085).
Reference for: spco-4: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: spco-4: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: spco-4: 1085. Trinci, A. P. J. 1973. Growth of wild type and spreading colonial mutants of Neurospora crassa in batch culture and on agar medium. Arch. Mikrobiol. 91:113-126.


To order, please copy the strain number into the Material Request Form 

Back to Strain Search Form

To the FGSC home page