FGSC #9372 Species: Neurospora crassa
Genotype: spco-4;fr
Alleles: R2367,B110
Linkage Group(s): VIIL,IL
Stock No. from Other Collection: OG7
Depositor of Strain: OG
Lesion: fr frost
Enzyme Name: glucose-6-phosphate dehydrogenase
Lesion Information for Marker:
      Linkage Group of fr: IL
      Markers Left of fr: ro-10 (18%)
      Markers Right offr: un-5 (6%)
Marker description or requirements:
      IL. Between ro-10 (18%) and un-5 (6%) (798, PB). (789)Delicate branching on agar surface and delicate aerial growth with no conidia (789). Multiple hyphal branching (382). Deficient in glucose- 6-phosphate dehydrogenase (as are col-2 and balmutants) (949, 952). Partially deficient in linolenic acid (115); morphology partially corrected by exogenous linolenic acid (892, 943). Low adenylate cyclase activity and low adenosine 3',5'-phosphate (943, 950). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Unlike cr-1, fr is not corrected morphologically by exogenous cyclic nucleotides (892, 951). Scott (943) reported that morphology is corrected by theophylline; Rosenberg and Pall (892) reported no correction by phosphodiesterase inhibitors. Cell wall analysis; photograph (112, 278, 946). Reduced amount of cell wall peptides (1165). Recessive in duplications (808). Female sterile. Both known alleles (B110 and R2499) revert to fr+.
Reference for: fr: 112. Brody, S. 1973. Metabolism, cell walls, and morphogenesis. In S. J. Coward (ed. ), Developmental regulation:aspects of celldifferentiation. Academic Press, New York.p. 107-154.
Reference for: fr: 115. Brody, S. and J. F. Nyc. 1970. Altered fatty acid distribution in mutants of N. crassa. J. Bacteriol. 104:780-786.
Reference for: fr: 278. De Terra, N. , and E. L. Tatum. 1963. A relationship between cell wall structure and colonial growth in Neurospora crassa. Am. J. Bot. 50:669-677.
Reference for: fr: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: fr: 779. Pall, M. L. , J. M. Trevillyan, and N. Hinman. 1981. Deficient cyclic adenosine 3',5'-monophosphate control in mutants of two genes ofNeurospora crassa. Mol. Cell. Biol. 1:1-8.
Reference for: fr: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: fr: 798. Perkins, D. D. 1972. An insertional translocation in Neurospora that generates duplications heterozygous for mating type. Genetics 71:25-51.
Reference for: fr: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: fr: 892. Rosenberg, G. , and M. L. Pall. 1979. Properties of two cyclic nucleotide-deficient mutants of Neurospora crassa. J. Bacteriol. 137:1140-1144.
Reference for: fr: 943. Scott, W. A. 1976. Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 73:2995-2999.
Reference for: fr: 946. Scott, W. A. 1979. Biochemical-genetic control of morphogenesis. In J. G. Scandalios (ed. ), Physiological genetics. AcademicPress, New York.p. 141-170.
Reference for: fr: 949. Scott, W. A. , and E. Mahoney. 1976. Defects of glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Neurospora and theirpleiotropic effects. Curr. Top. Cell Regul. 10:205-236.
Reference for: fr: 950. Scott, W. A. , N. C. Mishra, and E. L. Tatum. 1974. Biochemical genetics of morphogenesis in Neurospora. Brookhaven Symp. Biol. 25:1-18.
Reference for: fr: 951. Scott, W. A. and B. Solomon. 1975. Adenosine 3',5'-cyclic monophosphate and morphology in Neurospora crassa:drug-inducedalterations. J. Bacteriol. 122:454-463.
Reference for: fr: 952. Scott, W. A. , and E. L. Tatum. 1970. Glucose-6-phosphate dehydrogenase and Neurospora morphology. Proc. Natl. Acad. Sci. U. S. A. 66:515-522.
Reference for: fr: 1165. Wrathall, C. R. , and E. L. Tatum. 1974. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem. Genet. 12:59-68.
Lesion: spco-4 spreading colonial-4
Lesion Information for Marker:
      Linkage Group of spco-4: VIIL
      Markers Left of spco-4: Linked to do (<1%) and nic-3 (1%, probably to the left)
Marker description or requirements:
      VIIL. Linked to do (<1%) and nic-3 (1%, probably to the left) (816). Fine hyphae (382). Initially aconidial. Capable of conidiating on the surface of complete medium (D.D. Perkins, unpublished data). Hyphae extend faster within agar medium than on the surface, resulting in a dense hemispherical colony, most of which is embedded (1085).
Reference for: spco-4: 382. Garnjobst, L. , and E. L. Tatum. 1967. A survey of new morphological mutants in Neurospora crassa. Genetics 57:579-604.
Reference for: spco-4: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: spco-4: 1085. Trinci, A. P. J. 1973. Growth of wild type and spreading colonial mutants of Neurospora crassa in batch culture and on agar medium. Arch. Mikrobiol. 91:113-126.


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