FGSC #153 Mating Types: a Species: Neurospora crassa
Genotype: nic-3 met-7
Alleles: Y31881 4894
Linkage Group(s): VIIL VIIR
Stock No. from Other Collection: 7040
Depositor of Strain: DDP
Strain of Opposite Mating Type: 152
Reference: Perkins mimeo 1960 suppl. to 1959 genetics papers
Markers
Lesion: met-7 methionine-7
Enzyme Name: cystathionine-g-synthase
Lesion Information for Marker:
      Linkage Group of met-7: VIIR
      Markers Left of met-7: qa-2 (<1%), ars (<1%), and the centromere
      Markers Right ofmet-7: met-9 and wc-1(1-4%)
Marker description or requirements:
      VIIR. Right of qa-2 (<1%), ars (<1%), and the centromere (one second-division ascus in several hundred). Left of met-9 (10[-4]) and wc-1 (1 to 4%) (146, 725; M.E. Case, personal communication). (718; M.K. Allen, cited in references 718 and 789) Uses cystathionine, homocysteine, or methionine (718; N. H. Horowitz, cited in reference 1180). Lacks cystathionine-gamma-synthase (547) (Fig. 17). This enzyme is also lacking in the mutant met-3 (547). See met-3 for regulation. Apparently contiguous with met-9by coconversion. Flanking markers are recombined in most met-7+ met-9+ recombinants (725). Functionally distinct from the mutant met-9, which has active cystathionine-gamma-synthase (547) but cannot use homocysteine. No mutants lacking both functions have been isolated. Allele NM251 is suppressible by supersuppressor RN33 (same as ssu-1?) (725). Allele K79 is inseparable from reciprocal translocation T(I;VII)K79 (808).
Reference for: met-7: 146. Case. M. E. , and N. H. Giles. 1976. Gene order in the qa gene cluster of N. crassa. Mol. Gen. Genet. 147:83-89.
Reference for: met-7: 547. Kerr, D. S. , and M. Flavin. 1970. The regulation of methionine synthesis and the nature of cystathionine-synthase in Neurospora. J. Biol. Chem. 245:1842-1855.
Reference for: met-7: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: met-7: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: met-7: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: met-7: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: met-7: 1180. Zalokar, M. 1950. The sulfanomide-requiring mutant of Neurospora:threonine-methionine antagonism. J. Bacteriol. 60:191-203.
Lesion: nic-3 nicotinic acid-3
Lesion Information for Marker:
      Linkage Group of nic-3: VIIL
      Markers Left of nic-3: spco-4 (1%) and do (3%)
      Markers Right ofnic-3: thi-3 (9 to 27%) and csp-2 (16 to 22%)
Marker description or requirements:
      VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.
Reference for: nic-3: 96. Bonner, D. M. 1948. The identification of a natural precursor of nicotinic acid. Proc. Natl. Acad. Sci. U. S. A. 34:5-9.
Reference for: nic-3: 100. Bonner, D. M. , and C. Yanofsky. 1949. Quinolinic acid accumulation in the conversion of 3-hydroxyanthranilic acid to niacin inNeurospora. Proc. Natl. Acad. Sci. U. S. A. 35:576- 581.
Reference for: nic-3: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nic-3: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nic-3: 539. Kafer, E. , and E. Perlmutter. 1980. Isolation and genetic analysis of MMS-sensitive mus mutants of Neurospora. Can. J. Genet. Cytol. 22:535-552.
Reference for: nic-3: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: nic-3: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nic-3: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: nic-3: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: nic-3: 1168. Yanofsky, C. , and D. M. Bonner. 1950. Evidence for the participation of kynurenine as a normal intermediate in the biosynthesis of niacinin Neurospora. Proc. Natl. Acad. Sci. U. S. A. 36:167-176.

 

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