Genes from Neurospora crassa

New clones
Gene: GFP: Plasmid name: pMF272 Size in kb: 8.4
Resistance: amp
Depositor: MHF
Vector: pBM61
Notes:
Gene: hH1-GFP: Plasmid name: pMF280 Size in kb: 9.5
Resistance: amp
Depositor: MHF
Reference: Freitag et al. Fungal Genet Biol. 2004 Oct;41(10):897-910
Vector: pBM61
Notes:
Gene: Bml-GFP: Plasmid name: pMF309 Size in kb: 10.4
Resistance: amp
Depositor: MHF
Reference: Freitag et al. Fungal Genet Biol. 2004 Oct;41(10):897-910
Vector: pBM61
Gene: het-cGR: Plasmid name: het-c^GR Size in kb: 8.7
Resistance: CM
Depositor: Glass
Reference: Genetics 146:1299-1309
Vector: pCB1004
Gene: tol: Plasmid name: pTol Size in kb: 15.7
Resistance: cm
Depositor: Glass
Reference: Genetics
Vector: pCB1004
Gene: het-cPA: Plasmid name: het-cPA Size in kb: 8.8
Resistance: cm
Depositor: Glass
Reference: Genetics 146:1299-1309
Vector: pCB1004
Notes:
Gene: het-cOR: Plasmid name: het-cOR Size in kb: 8.9
Resistance: cm
Depositor: Glass
Reference: Genetics 143:1589-1600
Vector: pCB1004
Gene: acr-2: Plasmid name: pAC203 Size in kb: 9
Resistance: amp
Depositor: Nakashima
Reference: Biochim biophys Acta 1307:187-192
Vector: puc118
Gene: cys-9: Plasmid name: pCS907 Size in kb: 5.6
Resistance: amp
Depositor: Nakashima
Reference: Genetics 146:101-110
Vector: puc118
Gene: spe-3: Plasmid name: pTC102 Size in kb: 9
Resistance: amp
Depositor: Nakashima
Reference: J. Biol. Rhythm 13:452-
Vector: puc118
Gene: un-18: Plasmid name: pTS182 Size in kb: 11.8
Resistance: amp
Depositor: Nakashima
Reference: Mol Gen Genet 259:264- 271
Vector: puc118
Gene: thi-4: Plasmid name: pDC107(17:4B) Size in kb:
Resistance: amp
Depositor: Nakashima
Reference: Curr Genet 30:62-67
Vector: pDC107
Notes: This is a cosmid based on pDC107 that also confers benomyl resistance
Gene: Nc-ras2: Plasmid name: pTY6 Resistance: amp
Depositor: Murayama
Reference: MGG 254:427-432
Vector:
Notes:
Gene: Nc-ras2: Plasmid name: pTY7 Resistance: amp
Depositor: Murayama
Reference: MGG 254:427-432
Notes: Nc ras = smco-7
Gene: ncd-2: Plasmid name: pBncd-2 Size in kb: 4.3
Resistance: amp
Depositor: Kurihara
Reference: Flavins and Flavoproteins 1996 251-260
Vector: pBS SK-
Gene: hsp80: Plasmid name: pH807 Size in kb: 9.68
Resistance: amp
Depositor: M. Kapoor
Reference: Biochem Cell Biol 70:1365-1367
Vector: pUC13
Notes: 7.4 kB BamHI fragment (also called hspe-1) Accession # S58539
Gene: gdh-1: Plasmid name: pVG2 Size in kb: 6.89
Resistance: amp
Depositor: M. Kapoor
Reference: Biochem Cell Biol 71:205-219
Vector: pUC13
Notes: 4.2 Kb BamHI fragment
Gene: gdh-1: Plasmid name: pVG1 Size in kb: 8.95
Resistance: amp
Depositor: M. Kapoor
Reference: Biochem Cell Biol 71:205-219
Vector: pUC13
Notes: 6.26 BamHI fragment Accession number L20497
Gene: hsps-1: Plasmid name: pG6D Size in kb: 9
Resistance: amp
Depositor: M. Kapoor
Reference: J. Bacteriol 177:212-221
Vector: pUC18
Notes: hsp-70 plus 3 kb of flanking DNA Accession number U10443
Gene: gdh-1: Plasmid name: pGDH-x Size in kb:
Resistance: amp
Depositor: Kapoor
Gene: rgb-1: Plasmid name: pEY15 Resistance: amp
Depositor: O. Yarden
Reference: Mol Microbiol 31:197-
Vector: pBS
Gene: erg-3: Plasmid name: pSl2-1 Size in kb: 3.2
Resistance:
Depositor: D. Kasbekar
Reference:
Vector: pBS
Notes: erg-3 from Septoria lycopersici in pBluescriptKS as PstI fragment
Gene: erg-3: Plasmid name: pCHYG1 Resistance: amp
Depositor: D. Kasbekar
Reference: Microbiology 145:1443- 1451
Notes: pCH1-Hph in ref
Gene: erg-3: Plasmid name: pCHYG2 Resistance: amp
Depositor: D. Kasbekar
Reference: Microbiology 145:1443- 1451
Notes: pCH2-Hph in ref
Gene: erg-3: Plasmid name: pCHYG3 Resistance: amp
Depositor: D. Kasbekar
Reference: Microbiology 145:1443- 1451
Vector:
Notes: pCH3-Hph in ref
Gene: spe-2: Plasmid name: pSPE2 Resistance: amp
Depositor: RD
Reference:
Vector: pSP72
Gene: spe-2: Plasmid name: pSP6-spe-2 Resistance: amp
Depositor: RD
Reference:
Gene: TAD: Plasmid name: pTAD3.2 Size in kb: 13.5
Resistance: amp
Depositor: JAK
Reference:
Vector: pBS
Notes: Also freezerstock M7I9
Gene: mat-a: Plasmid name: pRATT05 Size in kb: 4.57
Resistance: amp
Depositor: RA
Reference:
Vector:
Notes:
Gene: inl: Plasmid name: pRATT19 Size in kb: 5.1
Resistance: amp
Depositor: RA
Gene: inl mat-a1: Plasmid name: pRATT25d Size in kb: 6.4
Resistance: amp
Depositor: RA
Gene: lux: Plasmid name: pLUC6 delta BS Size in kb: 8.4
Resistance: amp
Depositor:
Reference: FGB 38:327-332
Vector:
Notes: Fungal Genetics and Biology Volume 38, Issue 3 , April 2003, Pages 327-332
Gene: his-3: Plasmid name: pJHAM002 Size in kb:
Resistance: amp
Depositor: DWL
Reference: Curr Genet 43:17-23
Vector: pGEM13
Gene: his-3: Plasmid name: pJHAM003 Size in kb:
Resistance: amp
Depositor: DWL
Reference: Curr Genet 43:17-23
Vector: pGEM13
Gene: his-3: Plasmid name: pJHAM004 Size in kb:
Resistance: amp
Depositor: DWL
Reference: Curr Genet 43:17-23
Vector: pGEM13
Gene: his-3: Plasmid name: pJHAM005 Size in kb:
Resistance: amp
Depositor: DWL
Reference: Curr Genet 43:17-23
Vector: pGEM13
Gene: his-3: Plasmid name: pJHAM007 Size in kb:
Resistance: amp
Depositor: DWL
Reference: Curr Genet 43:17-23
Vector: pGEM13
Gene: 19s rRNA: Plasmid name: pHP2
Size in kb: 6.9
Resistance: tet
Depositor: AL
Vector: pBR322
Gene: 19s rRNA: Plasmid name: pBX17
Size in kb: 4.4
Resistance: amp
Depositor: AL
Vector: pSP64
Gene: VR telomere: Plasmid name: pNC36
Size in kb: 7.5
Resistance: amp
Depositor: M. Schechtman
Reference: Schechtman, Mol. Cell Biol. 7:3168 1987
Vector: pBR322
Notes: contains VR telomere, POGO BamHI linearizes
Click here for a restriction map of pNC36
Gene: al-1 (phytoene dehydrogenase): Plasmid name: pTJS342
Size in kb: 6.2
Resistance: amp
Depositor: T. Schmidhauser
Reference: Schmidhauser et al. 1990. Mol. Cell Biol. 10:5064-5070
Vector: pBS KS+
Gene: al-2 (phytoene synthetase): Plasmid name: pTJS542
Size in kb: 8.3
Resistance: amp
Depositor: T. Schmidhauser
Reference: Schmidhauser et al. 1994. J. Biol. Chem. 269:12060-12066
Vector: pBS KS+
Gene: am (NADP glutamate dehydrogenase): Plasmid name: pJR1
Size in kb: 11.8
Resistance: amp
Depositor: J. Kinsey
Reference: Kinsey and Rambosek 1984. Mol Cell Biol 4:117-122
Vector: pUC8
Click here to see a restriction map of the pJR1 insert
Gene: am (NADP glutamate dehydrogenase): Plasmid name: pJR2
Size in kb: 5.4
Resistance: amp
Depositor: J. Kinsey
Reference: Kinsey and Rambosek 1984. Mol Cell Biol 4:117-122
Vector: pUC8
Click here to see a restriction map of pJR2
Gene: arg-2 (carbamyl phosphate synthetase A): Plasmid name: pAB1
Size in kb: 6.1
Resistance: amp
Depositor: M. Sachs
Reference: Orbach et al 1990 JBC 265:10981
Vector: pDSP72
Notes: Single sites: BglII, ClaI, EcoRI, PvuII, PstI, SphI. arg-2 on 3.68 kb insert
Gene: asn: Plasmid name: pASN
Size in kb: 27.5
Resistance: amp
Depositor: F. Nargang
Vector: pSV50
Notes: doesn't appear to have a cosmid-sized insert
Gene: atp-1 (a- subunit of mitochondrial ATP synthase): Plasmid name : F1aG4
Size in kb: 9.0
Resistance: amp
Depositor: B. Bowman
Reference: Bowman and Knock 1992 Gene 114:157-163
Vector : pBS SK+
Notes : 6.0 Kb genomic EcoRV fragment. Genbank # M84191
Gene: atp-2 (B-subunit of mitochondrial ATP synthase): Plasmid name : F1B103A
Size in kb: 9.0
Resistance: amp
Depositor: B. Bowman
Reference: Bowman and Knock 1992 Gene 114:157-163
Vector : pBS SK+
Notes : 6.0 Kb genomic BamHI fragment. GenBank # M84192
Gene: BmlR (beta-tubulin): Plasmid name: pBT6
Size in kb: 5.8
Resistance: amp
Depositor: M. Orbach
Reference: Orbach et al 1986 Mol Cell Biol 6:2452-2461
Vector: pUC12
Click here to see a restriction map of pBT6
Gene: BmlR (beta-tubulin): Plasmid name: pSV50
Size in kb: 7.9
Resistance: amp
Depositor: S. Vollmer
Reference: Vollmer and Yanofsky 1986 PNAS 83:4869-4873
Notes: Cosmid, benomyl resistant. EcoR1 - 3.5 + 4.5
Click here to see a restriction map of pSV50
Gene: chs-2: Plasmid name: pAB22
Size in kb: 5.3
Resistance: amp
Depositor: O. Yarden
Reference: Beth Din and Yarden 1994 Microbiology 140:2189-2197
Vector: pSK-
Notes: 2.35 kb genomic EcoRI fragment in pSK-
Click here for a restriction map of pAB22
Gene: chs-2: Plasmid name: pAB23
Size in kb: 5.8
Resistance: amp
Depositor: O. Yarden
Reference: Beth Din and Yarden 1994 Microbiology 140:2189-2197
Vector: pSK-
Notes: 2.8 kb genomic KpnI-EcoRV fragment in pSK-
Click here for a restriction map of pAB23
Gene: ccg-1 (grg-1): Plasmid name: p2D30
Size in kb: 3.5
Resistance: amp
Depositor: S. Free
Reference: McNally and Free 1988. Curr Genet 14:545-551
Vector: pGEM3
Gene: cit-1 (Mitochondrial citrate syntase): Plasmid name: cit-1g
Size in kb: 8.0
Resistance: amp
Depositor: B. Bowman
Reference: Ferea et. al. 1994 Mol Gen Genet 242: 105-110 Vector: pBS SK+
Notes: 5.0 Kb genomic fragment
Click here to see a restriction map of cit-1g
Gene: cit-1 (Mitochondrial citrate syntase): Plasmid name: cit-1cDNA
Size in kb: 4.5
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Reference: Ferea et. al. 1994 Mol Gen Genet 242: 105-110
Gene: coI: Plasmid name: pRH70
Size in kb: 6.9
Resistance: amp
Depositor: AL
Vector: pBR322
Notes: HindIII 2.6 + 4.3
Gene: cot-1: Plasmid name: pOY18
Size in kb: 7.1
Resistance: amp
Depositor: O. Yarden
Reference: Yarden et al. 1992 EMBO J. 11:2159-2166
Vector: pSK-
Notes: 4.1 kb SmaI - EcoRI fragment in pSK-
Click here for a restriction map of pOY18
Gene: cpc-1: Plasmid name: pCPC1-2
Size in kb: 8.5
Resistance: amp
Depositor: Legerton
Reference: Paluh et al 1988 PNAS 85:3728-3732
Vector: pUC12
Notes: EcoR1 + SalI - 5.5 + 3.0.
Click here to see a restriction map of pCPC1-2 and pMO31
Gene: cpc-1: Plasmid name: pMO31
Size in kb: 5.6
Resistance: amp
Depositor: M. Orbach
Reference: Paluh et al 1988 PNAS 85:3728-3732
Vector: pUC18
Notes: EcoRI 1.6 + 3.7
Click here to see a restriction map of pCPC1-2 and pMO31
Gene: crp?: Plasmid name: p1717
Size in kb: 5.1
Resistance: amp
Depositor: S. Free
Reference: Tasaweh et al 1990 Nuc Acid Res in press
Vector: pBR322
Notes: EcoRI linearizes
Gene: cys-3: Plasmid name: pCYS3
Size in kb: 4.6
Resistance: amp
Depositor: Marzluf
Reference: Paietta et al 1987. MCB 7:2506-2511
Vector: pTZU18R
Click here to see a restriction map of pCYS3
Gene: cys-14 (sulfur permease II): Plasmid name: pJSK1
Size in kb: 10.4
Resistance: amp
Depositor: G. Marzluf
Reference: Ketter and Marzluf 1988. Mol. Cell. Biol 8:1504-1508
Vector: BS KS(+)
Gene: cyt-18 (tyrosyl-tRNA synthetase): Plasmid name: pRALcyt18
Size in kb: 10.5
Resistance: cap
Depositor: R. Akins
Reference: Akins and Lambowitz 1982 Cell
Vector: pRAL1
Gene: cyt-20 (valyl-tRNA synthetase): Plasmid name: pCyt20+
Size in kb: 6.6
Resistance: amp
Depositor: A. Kubelik
Reference:
Vector: pBS+
Notes: PvuII/NsiI fragment with wild type gene in pBS+
Gene: cyt-21 (mitochondrial ribosomal protein): Plasmid name: p297-24
Size in kb: 15.7
Resistance: cap
Depositor: A. Lambowitz
Reference: Kuiper et al 1988. JBC 263:2840-2847
Vector: pRAL1
Notes: BamHI 4.2 + 4.7 + 6.8
Click here for a restriction map of p297-24
Gene: cyt-22 (mitochondrial ribosomal protein): Plasmid name: pCYT22
Size in kb: 9.1
Resistance: cap
Depositor: A. Lambowitz
Reference: Kubelik, A. 1989. Ph.D. thesis
Vector: pRAL1
Gene: eas (ccg-2): Plasmid name: pLW1dK
Size in kb: 4.9
Resistance: amp
Depositor: D. Bell-Pedersen
Reference: Bell-Pedersen et al 1992 Genes Develop 6:2382-2394
Vector: pBSK+
Gene: erg-3: Plasmid name: pKGP86
Size in kb: 5.1
Resistance: amp
Depositor: D. Kasbekar
Reference: Papavinasasundaram and Kasbekar 1994. J. Genet. 73:33-41
Vector: pBS KS
Gene: frq: Plasmid name: pCRM101
Size in kb: 16.5
Resistance: amp
Depositor: R. McClung
Reference: McClung et al 1989 Nature 339:558-562
Vector: pSV50
Click here for a restriction map of pCRM101
Gene: gla-2: Plasmid name:pLMN1
Size in kb:13
Resistance: amp
Depositor: D. Ebbole
Reference: FGN 43: 23-25
Gene: his-3 (histidinol dehydrogenase): Plasmid name: pNH60
Size in kb: 10.5
Resistance: amp
Depositor: Schechtman
Reference: Legerton and Yanofsky 1985 Gene 39:129-140
Vector: pRK9
Notes: EcoR1/BamH1 - 7.5 + 3.0.
Gene: his-6: Plasmid name: pVRTEL1
Size in kb: 44.7
Resistance: amp
Depositor: 5-4-89
Reference: Schechtman 1987 Molec Cell Biol 7:3168-3177
Vector: pSV50
Notes: cosmid containing VR telomere and his-6 gene
Click here for a restriction map of pVRTEL1
Gene: histones: Plasmid name: pNcH3H4
Size in kb: 11.2
Resistance: amp
Depositor: L. Woudt
Reference: Woudt et al 1983 Nucl. Acids Res. 11:5347-5360
Vector: pBR322?
Notes: 6.9 SalI frag inserted in pBR322(?) HindIII - 8.8 + 2.4
Gene: ilv-2 (a-keto-B-hydroxylacyl reductoisomerase): Plasmid name: pHS503
Size in kb: 7.0
Resistance: amp
Depositor: B. Bowman
Reference: Sista and Bowman, 1992 Gene 120:115-118 Vector: pBS SK+
Notes: 4.0 Kb genomic BamHI fragment
Click here to see a restriction map of pHS503
Gene: inl (myoinositol-1-phosphatase ): Plasmid name: pINL
Size in kb: 19.1
Resistance: cap
Depositor: AL
Reference: Akins and Lambowitz 1985. MCB 5:2272-2278
Vector: pRAL1
Notes: insert is 14.7
Gene: inl (myoinositol-1-phosphatase?): Plasmid name: pOKE01
Size in kb: 11.2
Resistance: amp
Depositor: J. Groteluschen
Reference: unpubl
Vector: pUC18
Gene: lys-4: Plasmid name: pSL4
Size in kb: 12.7
Resistance: amp
Depositor: S. Kang
Vector: puc18?
Notes: 10 kb HindIII frag from SV50 cosmid 4:1G into pUC18(?)
Gene: met-7 (cystathionine gamma synthase): Plasmid name: pMET7
Size in kb: 8.4
Resistance: amp
Depositor: M. Case
Reference: Crawford et al 1992 Gene 111:265-266
Vector: pUC19
Click here to see a restriction map of pMET7
Gene: met-10: Plasmid name: pMET10
Size in kb: 8.8
Resistance: amp
Depositor: S. Kang
Reference: Kang and Metzenberg 1990 MCB 10:5839-5848
Vector: pGEM3ZF+
Notes: 5.6 kb EcoR1 in pGEM-3ZF(+)
Gene: mtA-1 (mating type A): Plasmid name: pMTAG-2
Size in kb: 4.2
Resistance: amp
Depositor: L. Glass/J. Grotelus
Reference: Glass et al 1990 PNAS 87:4912-4916
Vector: Sk+
Click here to see a restriction map of pMTAG-2
Gene: mta-1 (mating type a): Plasmid name: pCSN4
Size in kb: 4.9
Resistance: amp
Depositor: C. Staben
Reference: Staben and Yanofsky 1990 PNAS 87:4917-4921
Vector: Sk+
Notes: suppplied by L. Glass
Click here to see a restriction map of pCSN4
Gene: mtr (neutral amino acid permease): Plasmid name: pCVN2.9
Size in kb: 5.6
Resistance: amp
Depositor: D. Stuart
Reference: Koo and Stuart 1991 Genome 34:644-651
Vector: pTZ18R
Gene: mtr (neutral amino acid permease): Plasmid name: pN846
Size in kb: 5.6
Resistance: amp
Depositor: D. Stuart
Reference: Koo and Stuart 1991 Genome 34:644-651
Vector: pTZ18R
Gene: nic-1: Plasmid name: pNIC-1
Size in kb: 10.7
Resistance: cap
Depositor: A. Lambowitz
Reference: Akins and Lambowitz 1985. MCB 5:2272-2278
Vector: pRAL1
Notes: insert is 6.4
Gene: nit-2: Plasmid name: pNIT2
Size in kb: 8.7
Resistance: amp
Depositor: G. Marzluf
Reference: Fu and Marzluf 1987 MCB 7:1691-1696
Vector: pUC8
Notes: EcoRI - 6.0 + 2.7
Click here to see a restriction map of pNIT2
Gene: nit-3: Plasmid name: NIT3
Size in kb: 8.2
Resistance: amp
Depositor: G. Marzluf
Reference: Fu and Marzluf 1987 PNAS 84:8243-8247
Vector: pBS
Click here to see a restriction map of the plasmid containing NIT3
Please note, this is not the pNIT3 described in Fu and Marzluf.
Gene: nmr: Plasmid name: pNMR
Size in kb: 6.5
Resistance: amp
Depositor: G. Marzluf
Reference: Fu et al 1988 MGG 214:74-79
Vector: BS KS(+)
Click here to see a restriction map of pNMR
Gene: nuc-1: Plasmid name: pEXN3.5
Size in kb: 6.8
Resistance: amp
Depositor: S. Kang
Reference: Kang and Metzenberg 1990 Mol. Cell. Biol. 10:5839
Vector: pGEM3Zf+
Notes: 3.6 XbaI-EcoRI fragment in XbaI-EcoRI sites of pGEM3Zf(+)
Click here to see a restriction map of pEXN3.5
Gene: pan-2: Plasmid name: pOKE102
Size in kb: 5.8
Resistance: amp
Depositor: J. Grotelueschen
Vector: pSP72
Gene: pep-4 (Proteinase-A): Plasmid name: pep-4 genomic
Size in kb: 6.80
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes:3.8 kb PstI, ATG 14 bases distal from Polylinker 1.
Click here to see a restriction map of pep4g
Gene: pep-4 (Proteinase-A): Plasmid name: pep-4 cDNA
Size in kb: 4.60
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: 1.6 kb EcoRI, Note, contains 75 bases of unidentified DNA at poly A tail.
Click here to see a restriction map of pep4c
Gene: pho-2 (alkaline phosphatase): Plasmid name: pL3
Size in kb: 7.2
Resistance: amp
Depositor: R. Metzenberg
Reference: in preparation
Vector: pGEM3Zf+
Notes: 4 kb PstI fragment in PstI site of pGEM3Zf(+)
Click here to see a restriction map of pL3
Gene: pho-4 (phosphate permease): Plasmid name: pBJ0004
Size in kb: 8.0
Resistance: amp
Depositor: B.J. Mann
Reference: Mann et al 1988 MCB 8:1376, 1989 Gene 83:281
Vector: pGEM1
Notes: EcoRI - ~ 6 + 2
Gene: pho-5 (phosphate permease): Plasmid name: pWV01
Size in kb: 5.6
Resistance: amp
Depositor: W. Versaw
Reference: Versaw 1995 Gene 153:135-139
Vector: pGEM7Zf+
Click here to see a restriction map of pWV01
Gene: pma-1 (plasma membrane ATPase): Plasmid name: pKH14
Size in kb: 7.0
Resistance: amp
Depositor: C. Slayman
Reference: Hager et al 1986. PNAS 83:7693-7697
Vector: pSP64
Notes: HindIII 4.0 + 3.0
Click here to see a restriction map of pKH14
Gene: pph-1: Plasmid name: pphCos1
Size in kb:
Resistance: amp
Depositor:O. Yarden
Vector: pMOcosX
Notes: from Orbach/Sachs Library
Gene: pph-1: Plasmid name:pEY24
Size in kb:~7 kb
Resistance: amp
Depositor: O. Yarden
Reference: Curr Genet 28:458-466
Vector: pBluescript SK-
Notes: genomic subclone
A restriction map of pEY24
Gene: preg: Plasmid name: pPREG2.3
Size in kb: 5.5
Resistance: amp
Depositor: S. Kang
Reference: Kang and Metzenberg 1993 Genetics 133:193-202
Vector: pGEM3Zf+
Notes: 2.3 kb SphI-EcoRV cDNA fragment cloned in SphI-SmaI sites on pGEM3Zf(+)
Click here to see a restriction map of pREG2.3
Gene: pyr-4 (orotidine 5'-monophosphate decarboxylase): Plasmid name: pFB6
Size in kb: 8.6
Resistance: amp
Depositor: A. Radford
Reference: Buxton and Radford 1983 MGG 190:403-405
Vector: pRK9
Notes: EcoRI linearizes
Gene: qa genes: Plasmid name: pMSK374
Size in kb: 11.5
Resistance: tet
Depositor: M. Case
Vector: pBR322
Notes: begins in qa-x, runs through qa-2 and qa-4, ends in qa-3 PstI - 4.3 + 7.2
Gene: qa genes: Plasmid name: pMSK363
Size in kb: 11.7
Resistance: tet
Depositor: M. Case
Reference:
Vector: pBR322
Notes: begins in qa-3, runs through qa-y, qa-1S, ends in qa-1F. PstI 4.3 + 7.4
Gene: qa-1F: Plasmid name: pQA1F
Size in kb: 6.0
Resistance: amp
Depositor: M. Case
Vector: pUC19
Notes: HindIII linearizes
Gene: qa-3,qay: Plasmid name: pMSK381
Size in kb: 11.9
Resistance: amp
Depositor: M. Case
Vector: pBR325
Notes: runs through qa-3 and qa-y, ends in qa-1S. BamHI 4.3 + 7.6
Gene: qa-x,qa2: Plasmid name: pMSK338
Size in kb: 9.2
Resistance: amp
Depositor: M. Case
Vector: pBR325
Notes: BamHI 4.3 + 3.2
Gene: sod-1 (Cu-Zn superoxide dismutase): Plasmid name: pCN101
Size in kb: 4.7
Resistance: amp
Depositor: D. Natvig
Reference: Chary et al. 1990 JBC 265:18961-18967
Vector: pUC18
Notes: in JM109, sod-1 + 1 kb upstream XbaI-HindIII piece in pUC18
Click here to see a restriction map of pCN101
Gene: spe-1 (ornithine decarboxylase): Plasmid name: pGS1
Size in kb: 7.9
Resistance: amp
Depositor: R.H. Davis
Reference: Williams et al. 1992 Mol. Cell Biol. 12:347-359
Vector: pBS
Notes: 4.95 kb HindIII fragment in pBS
Click here for a map of pGS1
Gene: T (tyrosinase): Plasmid name: pTYR103
Size in kb: 5.6
Resistance: amp
Depositor: S. Free
Reference: Kothe et al 1993 FGN 40
Vector: pBSIISK-
Notes: From Vollmer-Yanofsky cosmid 32:A9
Click here to see a restriction map of pTYR103
Gene: trp-1 (anthranilate synthetase): Plasmid name: pNC2
Size in kb: 9.3
Resistance: amp
Depositor: C. Yanofsky
Reference: Schechtman and Yanofsky 1983 J Mol Appl Genet 2:83-99
Vector: pRK9
Notes: HindIII linearizes.
Gene: trp-3 (tryptophan synthetase): Plasmid name: pDB1
Size in kb: 8.1
Resistance: amp
Depositor: C. Yanofsky
Vector: pBR322
Notes: 3.8 kb XhoI fragment from pSV50 5:4G. EcoRI, BamHI linearize
Gene: un-3-: Plasmid name: pUN3
Size in kb: 6.6
Resistance: amp
Depositor: A. Lambowitz
Vector: pBS-
Notes: PvuII/NsiI fragment containing un-3 mutation in pBS-
Gene: vma-1 (Vacuolar ATPase, genomic): Plasmid name: vma-1g
Size in kb: 12.0
Depositor: B. Bowman
Vector: pBluescript SK(+)
Reference: Bowman et. al. 1988 J Biol Chem 263:13994-14001
Notes: 9.0 kb genomic EcoRI DNA fragment encoding the 67 kDa subunit. Genbank # J03955
Click here to see a restriction map of vma-1g
Gene: vma-1 (Vacuolar ATPase, cDNA): Plasmid name: pRB34
Size in kb: 5.2
Depositor: B. Bowman
Reference: Bowman et. al. 1988 J Biol Chem 263:13994-14001
Vector: pBluescript SK (-)
Notes: 2.2 kb EcoRI cDNA fragment encoding the 67 kDa subunit. Genbank # J03955
Click here to see a restriction map of pRB34
Gene: vma-2 (Vacuolar ATPase, genomic): Plasmid name: pBB4
Size in kb: 5.8
Depositor: B. Bowman
Reference: Bowman et. al. 1988 J. Biol Chem 263:14002-14007
Vector: pBluescript SK(+)
Notes: 2.8 Kb KpnI genomic DNA fragment. Encodes the 57 kDa subunit.
Genbank #: J03956
Click here to see a restriction map of pBB4
Gene: vma-2 (Vacuolar ATPase, genomic): Plasmid name: pMW1
Size in kb: 6.5
Depositor: B. Bowman
Reference: Bowman et. al. 1988 J. Biol Chem 263:14002-14007
Vector: pBluescript SK(+)
Notes: 3.5 Kb genomic BamHI to ClaI DNA fragment including upstream sequence. Encodes the 57 kDa subunit.
Genbank #: J03956
Click here to see a restriction map of pMW1
Gene: vma-2 (Vacuolar ATPase, cDNA): Plasmid name: pRB30
Size in kb:5.0
Depositor: B. Bowman
Reference: Bowman et. al. 1988 J. Biol Chem 263:14002-14007
Vector: pBluescript SK(+)
Notes:2.0 Kb EcoRI cDNA fragment encodes the 57 kDa subunit of VM ATPase.
Genbank # : J03956
Click here to see a restriction map of pRB30
Gene: vma-3 (Vacuolar ATPase, genomic): Plasmid name: pHS214
Size in kb: 6.0
Depositor: B. Bowman
Reference: Sista et. al. 1994 Mol Gen Genet 243:82-90
Vector: pBluescript SK(+)
Notes:3.0 kb PstI SalI fragment encoding the 16 kDa proteolipid subunit
Genbank #: L07105
Click here to see a restriction map of pHS214
Gene: vma-3 (Vacuolar ATPase, cDNA): Plasmid name: pHS171
Size in kb: 6.0
Depositor: B. Bowman
Reference: Sista et. al. 1994 Mol Gen Genet 243:82-90
Vector: pBluescript SK(+)
Notes: vma-3 and ilv-2 cDNAs fused at their 5' ends; poly A tail at both ends. 3 KB EcoRI fragment.
Genbank #: L07105
Click here to see a descriptive map of pHS171
Gene: vma-4 (Vacuolar ATPase, genomic): Plasmid name: pAS4A
Size in kb: 5.5
Depositor: B. Bowman
Vector: pUC13
Notes:2.9 Kb genomic PstI fragment encoding subunit E of the vacuolar ATPase
Click here to see a restriction map of pAS4A
Gene: vma-4 (Vacuolar ATPase, cDNA): Plasmid name: pAS30
Size in kb: 4.4
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: fusion of two EcoRI fragments. The first 148 nt are of unknown origin
Click here to see a restriction map of pAS30
Gene: vma-6 (Vacuolar ATPase, genomic): Plasmid name: vma-6g
Size in kb: 10.5
Depositor: B. Bowman
Vector: pBS SK+
Notes: 5.3 Kb genomic PstI fragment. Also contains all of the the LAMR gene
Click here to see a restriction map of vma-6g
Gene: vma-6 (Vacuolar ATPase, cDNA): Plasmid name: vma-6c
Size in kb: 4.5
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: 1.5 Kb cDNA EcoRI fragment. vma-6 encodes the 40 kD subunit of V-type ATPase
Click here to see a restriction map of vma-6c
Gene:vph-1 (Vacuolar ATPase, genomic): Plasmid name: pRB103A
Size in kb: 7.20
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: 4.2 Kb genomic KpnI fragment. vph-1 encodes the 100 kD subunit of Vacuolar ATPase
Click here to see a restriction map of pRB103A
Gene:vph-1 (Vacuolar ATPase, cDNA): Plasmid name: pRBS
Size in kb: 5.80
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: 2.8 Kb EcoRI fragment. vph-1 encodes the 100 kD subunit of Vacuolar ATPase
Click here to see a restriction map of pRBS
Gene:vph-1 (genomic): Plasmid name: pRB103A
Size in kb: 7.20
Resistance: amp
Depositor: B. Bowman
Vector: pBS SK+
Notes: 4.2 Kb KpnI fragment. vph-1 encodes the 100 kD subunit of Vacuolar ATPase
Click here to see a restriction map of pRB103A
Mitochondrial DNAs
Gene: mtDNA: Plasmid name: pEco2
Size in kb: 14.5
Resistance: amp
Depositor: A. Lambowitz
Vector: pBR322
Notes: EcoRI 10.6 + 4.3
Gene: mtDNA: Plasmid name: pEco4
Size in kb: 9.1
Resistance: amp
Depositor: A. Lambowitz
Vector: pBR322
Notes: EcoRI 5.2 + 4.3
Gene: mtDNA: Plasmid name: pEco6
Size in kb: 6.3
Resistance: amp
Depositor: A. Lambowitz
Vector: pBS
Notes: EcoRI 3.3 + 3.2
Gene: mtDNA: Plasmid name: pHPR4a
Size in kb: 15.0
Resistance: tet
Depositor: A. Lambowitz
Vector: pBR322
Gene: mtDNA: Plasmid name: pND3
Size in kb: 0.0
Resistance: amp
Depositor: A. Lambowitz
Vector: pBS
pUCB plasmids from John Taylor: These plasmids contain EcoRI fragments of N. crassa mtDNA cloned in pBR325 and grown in the E. coli strain HB101.
Click here for a description of these EcoRI fragments.
Gene: mtDNA: Plasmid name: pUCB3
Size in kb: 14.8
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB4
Size in kb: 10.8
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB5
Size in kb: 9.5
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB6
Size in kb: 9.1
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB7a
Size in kb: 8.9
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB7b
Size in kb: 8.8
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB8
Size in kb: 8.3
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB9
Size in kb: 8.0
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB10
Size in kb: 7.9
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325
Gene: mtDNA: Plasmid name: pUCB11
Size in kb: 6.6
Resistance: amp
Depositor: J. Taylor
Reference: Taylor and Smolich 1985 Curr Genet 9:597-603
Vector: pBR325

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Last modified 11/98 KMC

Genes from Neurospora crassa

Mitochondrial DNAs

pUCB plasmids from John Taylor