Aspergillus nidulans pyrE and
A. Aleksenko1, H.N. Arst, Jr.2 and A.J. Clutterbuck3, 1Center for Process Biotechnology, DTU, Lyngby, 2800 Denmark, 2Dept of Infectious Diseases, Imperial College School of Medicine, Hammersmith Hospital, Ducane Road, London W12 0NN, England, 3Anderson College, University of Glasgow, Glasgow G11 6NU, Scotland, U.K.
Sequencing of a gene cloned by complementation of a pyr mutant in Glasgow strain G190 shows this mutant to be pyrE, encoding dihydroorotate dehydrogenase. This discovery necessitates a revision of the genetic map derived from crosses with this strain.
anA 9.3 pyrE 6.2 adG 9.0 pyrF 6.5 luA
Subsequent work involved Cambridge strain 595 (= Glasgow G190, and FGSC A721), original genotype: anA1 pyrE8 luA1 yA2; cnxH5. Arst 1988 (Mol. Gen. Genet. 213: 545-547) mapped the pyr mutation in this strain:
anA 10.0 adG 0.6 davA 0.4 pyr? 5.0 luA 2.0 ornD
(9/102, 14/128) (19/3088) (12/2728) (6/74, 3/102, 4/82) (3/149)
From this map it was concluded that the mutation in this strain must be pyrF rather than pyrE, and both Glasgow and FGSC strains designations were "corrected" accordingly. However, the "pyrF" gene that was cloned by complementation of this mutation (Aleksenko et al. 1999 Mol. Microbiol. 33:599-611) has now been sequenced and proves to encode dihydroorotate dehydrogenase, so the original description of the strain as carrying pyrE8 was correct, and the fault lies in the mapping.
Arst (1988) used two crosses with the disputed strain to map these mutants, all data being obtained by selection of a class of recombinants and subsequent analysis of outside markers, which gave a consistent order in every case. On the other hand, Palmer and Cove gave no details of their crosses or number of progeny analysed. It should also be noted that Palmer and Cove's map position for pyrD has also been revised in subsequent work ( Aleksenko et al. 1999 Mol. Microbiol. 33:599-611), and that, pyrG, which was reported to be unlinked to galD, is now agreed to be quite close to it (Kafer and May 1988 Fungal Genet. Newslett. 35:13-15).
It therefore seems prudent to accept the Arst data and conclude that pyrE maps between davA and luA, while the position of pyrF is unknown. Unfortunately, no authentic pyrF mutant strains are now available - unless any reader knows better.
Return to the FGN 47 page
Return to the main FGN page
Return to the FGSC main page
Last modified 8/4/00 KMC