Cryptococcus at the FGSC

Wild type strains



Deletion Sets

2016 Bahn set of 5 plates (MS Excel 2003)

Please see Lee, Kyung-Tae, et al. "Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans." Nature Communications 7 (2016).
https://www.ncbi.nlm.nih.gov/pubmed/27677328
Received in November 2016 and anticipated release December 12-15, 2016.


2015 Bahn set of 4 plates (MS Excel 2003)

Please see Jung KW, Yang DH, Maeng S, Lee KT, So YS, et al. (2015) Systematic functional profiling of transcription factor networks in Cryptococcus neoformans. Nat Comms 6: 6757.
http://www.ncbi.nlm.nih.gov/pubmed/25849373


2016 Madhani plates (MS Excel 2003)

The new 2016 set was received at the FGSC early in July 2016 and is available as complete set of 20 plates or individual clones.

2015 Madhani plates (MS Excel 2003)

The new 2015 set was received at the FGSC early in March 2015 and is available as complete set of 22 plates or individual clones.

A feedback form is available for clients to report on strain issues

Regarding the 2015 sets, the UCSF team has asked that we post the following statement about acknowledgement of the resource:
These knockout strains have been made freely available ahead of publication to the scientific community. It is requested that NIH funding (R01AI100272) for this project and the Madhani laboratory be acknowledged in any publication that uses this resource. Individuals interested in performing systematic, high-throughput analysis are asked to contact the Madhani lab to ensure coordination with ongoing efforts (hitenmadhani@gmail.com).

All of the 2015 deletion mutants are nourseothricin (NAT) resistant and can be propagated on YPD (or any other standard yeast media such as YNB or Sabouraud) supplemented with 0.1mg/ml NAT. We routinely use 0.075mg/ml NAT as we have found that specific activity of NAT varies from batch to batch. The parental strain KN99alpha is derived from H99 clinical isolate of C. neoformans Serotype A grubii and is a prototroph.

Chun and Madhani. 2010. Methods in Enzymology 470: p797 (PMID 20946836) has more information on how the knockout constructs are generated and how to propagate the strains.

In the figure, primer "1" corresponds to W1 in the spreadsheet , "2" to W2 etc.
For folks trying to determine what region is deleted at each locus, they can match the sequence of W2 and W5 (uppercase letters) to the genome. The sequence in between W2 and W5 has been replaced with the drug cassette. The lowercase letters of W2 and W5 primers are the sequences homologous to the drug cassette.

This database includes primer sequences


2008 Madhani, set of 14 plates
(More information)



2007 J. Lodge: Strains at the FGSC in 2 plates (A and B)

Background Data

 

    Deletion mutants are distributed as frozen cultures in 96- well plates or on agar solidified medium (additional fees apply).
    We ask $100 per plate plus shipping. International destinations require special clearance and pre-approval.
    Not all strains listed in background data were sent to the FGSC.

        

 


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8/26/16