The preferred source is the Fungal Genetics Stock Center (FGSC), Humboldt State University, Arcata, CA 95521. FGSC specializes in Neurospora stocks for research. Nearly 4,000 Neurospora stocks are maintained, including key alleles at most of the known loci. These are available free of charge to individuals and nonprofit institutions. Stock lists are published biennially in the Neurospora Newsletter under the following headings: single mutants; multiple mutants; nonchromosomal mutants; wild type and wild-collected strains (representing all known species); chromosome rearrangements; reference strains, testers, and stocks for special purposes. The last category includes linkage testers, standard N. crassa wild types and mating type testers, species diagnostic testers and reference strains, heterokaryon incompatibility testers, strains for mutant enrichment and replication, strains that produce only microconidia, strains for obtaining protoplasts or spheroplasts, Spore-killer testers, strains for mutagenesis, nonsense suppressors, and transport mutants.
The American Type Culture Collection maintains about 200 Neurospora stocks.
Information on all the known gene loci of N. crassa is gathered in a compendium. Over 500 loci have been mapped to the seven linkage groups. Linkage maps have been revised, resolving many ambiguities of gene order. A separate entry for each locus gives documented information on linkage, phenotype, biochemistry, scoring, interactions with other loci, regulation, fertility, stability, complementation, fine structure, and uses in research. Entries are also given for the nucleolus organizer, for individual centromeres and chromosome tips, and for chromosome rearrangements that have been used in mapping. Diagrams of biosynthetic and catabolic pathways show sites of gene action. Brief summaries are given of the history of Neurospora research and of current genetic nomenclature and stock sources.
We are greatly indebted to over 100 investigators who have checked entries for accuracy and completeness. They corrected numerous errors and remedied important omissions. We regret that they cannot be acknowledged by name. Major help and encouragement have been given by Rowland H. Davis, Stuart Brody, Roy W. Harding, Robert L. Metzenberg, and Orest M. Mylyk. Investigators who have communicated unpublished information are named in the text.
Work in our laboratories has been supported by Public Health Service grants Al-01462 and K6-GM-4899 from the National Institutes of Health (D.D.P.) and by U.K. Science and Engineering Research Council grants GR/A/64655 and GR/Bn8526 (A.R.).
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Last modified 4/24/96 KMC