Candida resources at the FGSC

 
Knockout sets from Aaron Mitchell
December 2008

Green
1    CJN TF Homozygous mutants

2    2nd set TF Homozygous mutants 1
3    2nd set TF Homozygous mutants 2
 
Yellow
4    VB GKO Plate 1
5    VB GKO Plate 2
6    VB GKO Plate 3
7    VB GKO Plate 4
8    VB GKO Plate 5
Additional information may be found in this spreadsheet from the Mitchell lab.

 

Blue
9     Dominique Sanglard TF mutants
10   SFY Homozygous mutants plate 1
 
Red
11    CWP Homozygous mutants plate 1
12    CWP Homozygous mutants plate 2
 
Orange
13    Misc Homozygous mutants plate 1

12/18/09

New Kinase sets available

 

A marker-matched control is present in most of the plates. It is called strain DAY286


Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1.
Rauceo JM, Blankenship JR, Fanning S, Hamaker JJ, Deneault JS, Smith FJ, Nantel A, Mitchell AP. Mol Biol Cell. 2008 Jul;19(7):2741-51.
PMID: 18434592

Requirement for Candida albicans Sun41 in biofilm formation and virulence.
Norice CT, Smith FJ Jr, Solis N, Filler SG, Mitchell AP. Eukaryot Cell. 2007 Nov;6(11):2046-55. Epub 2007 Sep 14.
PMID: 17873081

Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p.
Nobile CJ, Mitchell AP. Curr Biol. 2005 Jun 21;15(12):1150-5.
PMID: 15964282

Control of the C. albicans cell wall damage response by transcriptional regulator Cas5.
Bruno VM, Kalachikov S, Subaran R, Nobile CJ, Kyratsous C, Mitchell AP. PLoS Pathog. 2006 Mar;2(3):e21.
 

Additional information may be obtained from apm1@andrew.cmu.edu

 

 

 

 

Knockout set from Oliver Homann
January 2010

Deletion strains were created for 165 different transcriptional regulators, and in most cases two independently derived knockouts were created for each transcriptional regulator.
They are designated 'X' and 'Y' (e.g. TF021-X and TF021-Y).

In total 4 plates are available

A description of the deletions

A spreadsheet of listing the plate contents and primers used in strain construction

The deletions are also described in Homann OR et al. A phenotypic profile of the Candida albicans regulatory network. PLoS Genet. 2009 Dec;5(12)e1000783

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1/27/10 KMC